PDF(Pubmed)
Abstract：
OBJECTIVE: Exercise-induced muscle damage (EIMD) results in the generation of reactive oxygen species (ROS), but little is known about the temporal profile of change in ROS post-EIMD and how ROS levels relate to the onset of and recovery from EIMD. Our primary aim was to examine the effect of EIMD on the pattern of change in the blood level of thiol-oxidised albumin, a marker of oxidative stress.
METHODS: Seven male participants were subjected on separate days to eccentric muscle contraction to cause EIMD or a no-exercise condition. After each session, the participants collected daily dried blood spots to measure thiol-oxidised albumin and returned to the laboratory every 2 days for the assessment of indirect markers of EIMD, namely maximal voluntary contraction (MVC), delayed onset muscle soreness (DOMS), creatine kinase (CK), and myoglobin.
RESULTS: Eccentric exercise resulted in a significant decrease in MVC and increase in DOMS, CK, myoglobin, and thiol-oxidised albumin with the latter reaching above baseline level within 24-48 h post-exercise. All the markers of EIMD returned to baseline level within 6 days post-exercise, but not the level of thiol-oxidised albumin which remained elevated for 10 days after exercise. There was a moderate correlation between changes in thiol-oxidised albumin and DOMS, but no significant relationship between any other markers of muscle damage.
CONCLUSIONS: The levels of thiol-oxidised albumin increase in response to EIMD and remain elevated for several days post-exercise. The temporal pattern of change in the level of thiol-oxidised albumin suggests that this may be a useful biomarker of muscle repair post-EIMD.
METHODS: Seven male participants were subjected on separate days to eccentric muscle contraction to cause EIMD or a no-exercise condition. After each session, the participants collected daily dried blood spots to measure thiol-oxidised albumin and returned to the laboratory every 2 days for the assessment of indirect markers of EIMD, namely maximal voluntary contraction (MVC), delayed onset muscle soreness (DOMS), creatine kinase (CK), and myoglobin.
RESULTS: Eccentric exercise resulted in a significant decrease in MVC and increase in DOMS, CK, myoglobin, and thiol-oxidised albumin with the latter reaching above baseline level within 24-48 h post-exercise. All the markers of EIMD returned to baseline level within 6 days post-exercise, but not the level of thiol-oxidised albumin which remained elevated for 10 days after exercise. There was a moderate correlation between changes in thiol-oxidised albumin and DOMS, but no significant relationship between any other markers of muscle damage.
CONCLUSIONS: The levels of thiol-oxidised albumin increase in response to EIMD and remain elevated for several days post-exercise. The temporal pattern of change in the level of thiol-oxidised albumin suggests that this may be a useful biomarker of muscle repair post-EIMD.
摘要：
目的：运动诱发的肌肉损伤（EIMD）导致产生活性氧（ROS），但对EIMD后ROS变化的时间分布以及ROS水平与EIMD发病和恢复的关系知之甚少。我们的主要目的是检查EIMD对硫醇氧化白蛋白血液水平变化模式的影响，氧化应激的标志.
方法：7名男性参与者在不同的日子里接受偏心肌肉收缩，导致EIMD或无运动状态。每次会议结束后,参与者每天收集干燥的血斑以测量硫醇氧化白蛋白，每2天返回实验室评估EIMD的间接标志物。即最大自愿收缩(MVC)，延迟性肌肉酸痛（DOMS），肌酸激酶（CK），和肌红蛋白.
结果：偏心运动导致MVC显着下降，DOMS增加，CK,肌红蛋白,和硫醇氧化白蛋白，后者在运动后24-48小时内达到基线水平以上。EIMD的所有标志物在运动后6天内恢复到基线水平，而不是运动后10天保持升高的硫醇氧化白蛋白水平。硫醇氧化白蛋白和DOMS的变化之间存在中等相关性，但其他任何肌肉损伤标志物之间没有显著关系。
结论：硫醇氧化白蛋白水平在EIMD反应中增加，并在运动后几天保持升高。巯基氧化白蛋白水平变化的时间模式表明，这可能是EIMD后肌肉修复的有用生物标志物。
方法：7名男性参与者在不同的日子里接受偏心肌肉收缩，导致EIMD或无运动状态。每次会议结束后,参与者每天收集干燥的血斑以测量硫醇氧化白蛋白，每2天返回实验室评估EIMD的间接标志物。即最大自愿收缩(MVC)，延迟性肌肉酸痛（DOMS），肌酸激酶（CK），和肌红蛋白.
结果：偏心运动导致MVC显着下降，DOMS增加，CK,肌红蛋白,和硫醇氧化白蛋白，后者在运动后24-48小时内达到基线水平以上。EIMD的所有标志物在运动后6天内恢复到基线水平，而不是运动后10天保持升高的硫醇氧化白蛋白水平。硫醇氧化白蛋白和DOMS的变化之间存在中等相关性，但其他任何肌肉损伤标志物之间没有显著关系。
结论：硫醇氧化白蛋白水平在EIMD反应中增加，并在运动后几天保持升高。巯基氧化白蛋白水平变化的时间模式表明，这可能是EIMD后肌肉修复的有用生物标志物。
