PDF(Pubmed)
Abstract：
The family of phosphatidylethanolamine-binding proteins (PEBPs) participates in various plant biological processes, mainly flowering regulation and seed germination. In cucurbit crops, several PEBP genes have been recognized to be responsible for flowering time. However, the investigation of PEBP family members across the genomes of cucurbit species has not been reported, and their conservation and divergence in structure and function remain largely unclear. Herein, PEBP genes were identified from seven cucurbit crops and were used to perform a comparative genomics analysis. The cucurbit PEBP proteins could be classified into MFT, FT, TFL, and PEBP clades, and further, the TFL clade was divided into BFT-like, CEN-like, and TFL1-like subclades. The MFT-like, FT-like, and TFL-like proteins were clearly distinguished by a critical amino acid residue at the 85th position of the Arabidopsis FT protein. In gene expression analysis, CsaPEBP1 was highly expressed in flowers, and its expression levels in females and males were 70.5 and 89.2 times higher, respectively, than those in leaves. CsaPEBP5, CsaPEBP6, and CsaPEBP7 were specifically expressed in male flowers, with expression levels 58.1, 17.3, and 15.7 times higher, respectively, than those of leaves. At least five CsaPEBP genes exhibited the highest expression during the later stages of corolla opening. Through clustering of time-series-based RNA-seq data, several potential transcription factors (TFs) interacting with four CsaPEBPs were identified during cucumber corolla opening. Because of the tandem repeats of binding sites in promoters, NF-YB (Csa4G037610) and GATA (Csa7G64580) TFs appeared to be better able to regulate the CsaPEBP2 and CsaPEBP5 genes, respectively. This study would provide helpful information for further investigating the roles of PEBP genes and their interacting TFs in growth and development processes, such as flowering time regulation in cucurbit crops.
摘要：
磷脂酰乙醇胺结合蛋白（PEBP）家族参与各种植物生物过程，主要是开花调节和种子萌发。在葫芦作物中，已经认识到几种PEBP基因负责开花时间。然而,对瓜类物种基因组中PEBP家族成员的调查尚未见报道，它们在结构和功能上的保守和分歧在很大程度上仍然不清楚。在这里，从7种葫芦作物中鉴定出PEBP基因，并将其用于进行比较基因组学分析。瓜类PEBP蛋白可分为MFT,FT,TFL,和PEBP进化枝，进一步,TFL进化枝被分成类似BFT，CEN-like,和类似TFL1的子分支。像MFT一样，FT-like,和TFL样蛋白通过拟南芥FT蛋白的第85位的关键氨基酸残基明显区分。在基因表达分析中，CsaPEBP1在花中高表达，其在女性和男性中的表达水平分别高70.5和89.2倍，分别,比树叶里的。CsaPEBP5，CsaPEBP6和CsaPEBP7在雄花中特异性表达，表达水平高58.1、17.3和15.7倍，分别,比那些叶子。在花冠开放的后期，至少有五个CsaPEBP基因表现出最高的表达。通过对基于时间序列的RNA-seq数据进行聚类，在黄瓜花冠开放过程中，鉴定了几种与四个CsaPEBPs相互作用的潜在转录因子（TFs）。由于启动子中结合位点的串联重复，NF-YB(Csa4G037610)和GATA(Csa7G64580)TFs似乎能更好地调控CsaPEBP2和CsaPEBP5基因,分别。这项研究将为进一步研究PEBP基因及其相互作用的TFs在生长发育过程中的作用提供有用的信息。如瓜类作物的开花时间调节。
