PDF(Pubmed)
Abstract：
Chenopodium quinoa Willd. is rich in phenolic compounds and exhibits diverse biological activities. Few studies have focused on the effect of colored quinoa\'s phenolic profile on potential biological activity. This study used a UPLC-MS/MS-based metabolomic approach to examine the quinoa phenolics and their association with in vitro antioxidant and hypoglycemic properties. In total, 430 polyphenols, mainly phenolic acids, flavonoids, and flavonols, were identified. Additionally, 121, 116, and 148 differential polyphenols were found between the white and black, white and red, and black and red comparison groups, respectively; 67 polyphenols were screened as shared key differential metabolites. Phenylalanine, tyrosine, and the biosynthesis of plant secondary metabolites were the main differently regulated pathways. Black quinoa had better total phenolic contents (643.68 mg/100 g DW) and antioxidant capacity, while white quinoa had better total flavonoid contents (90.95 mg/100 g DW) and in vitro α-amylase (IC50 value of 3.97 mg/mL) and α-glucosidase (IC50 value of 1.08 mg/mL) inhibition activities. Thirty-six polyphenols, including epicatechin and linarin, etc., were highly correlated with in vitro antioxidant activity, while six polyphenols, including tiliroside and chrysoeriol, etc., were highly correlated with in vitro hypoglycemic activity. This study may provide important information for colored quinoa resources to develop their healthy food applications.
摘要：
藜麦。富含酚类化合物，表现出多样化的生物活性。很少有研究关注有色藜麦的酚类成分对潜在生物活性的影响。这项研究使用基于UPLC-MS/MS的代谢组学方法来检查藜麦酚类物质及其与体外抗氧化和降血糖特性的关联。总的来说,430多酚，主要是酚酸，黄酮类化合物，和黄酮醇，已确定。此外,在白色和黑色之间发现了121、116和148种差异多酚，白色和红色,黑色和红色比较组，分别筛选出67种多酚作为共有的关键差异代谢产物。苯丙氨酸,酪氨酸,植物次生代谢产物的生物合成是主要的不同调控途径。黑藜麦具有较好的总酚含量(643.68mg/100gDW)和抗氧化能力,而白藜麦具有较好的总黄酮含量（90.95mg/100gDW）和体外α-淀粉酶（IC50值为3.97mg/mL）和α-葡萄糖苷酶（IC50值为1.08mg/mL）抑制活性。36种多酚,包括表儿茶素和linarin,等。,与体外抗氧化活性高度相关，而六种多酚，包括利洛苷和大黄二醇，等。,与体外降血糖活性高度相关。本研究可为有色藜麦资源开发其健康食品应用提供重要信息。
