Abstract：
OBJECTIVE: This study investigated phenotypic and genotypic antimicrobial resistance profiles of Vibrio strains identified from Mytilus galloprovincialis farmed for human consumption in the Adriatic Sea Central Italy.
RESULTS: A total of 475 mussels (M. galloprovincialis) were involved in the present study, and culture-dependent microbiological methods permitted to identify a total of 50 Vibrio strains that were tested for antibiotic susceptibility followed by the genetic determinant detections. Antibiograms showed resistance against ampicillin (36.0%), amoxicillin-clavulanic acid (30.0%), gentamycin (14.0%), and imipenem (18.0%). Biomolecular assays amplified a total of 264 antibiotic resistance genes harbored by both susceptible and resistant Vibrio species. Among resistance genes, aacC2 (62.0%) and aadA (58.0%) for aminoglycosides, blaTEM (54.0%) for beta-lactams, qnrS (24.0%) for quinolones, tetD (66.0%) for tetracyclines, and vanB (60.0%) for glycopeptides were mainly amplified by PCR assays.
CONCLUSIONS: Vibrio genus is involved in the antibiotic resistance phenomenon diffusion in the aquatic environments, as demonstrated by the harboring of many genetic determinants representing a kind of genetic \"dark world\".
RESULTS: A total of 475 mussels (M. galloprovincialis) were involved in the present study, and culture-dependent microbiological methods permitted to identify a total of 50 Vibrio strains that were tested for antibiotic susceptibility followed by the genetic determinant detections. Antibiograms showed resistance against ampicillin (36.0%), amoxicillin-clavulanic acid (30.0%), gentamycin (14.0%), and imipenem (18.0%). Biomolecular assays amplified a total of 264 antibiotic resistance genes harbored by both susceptible and resistant Vibrio species. Among resistance genes, aacC2 (62.0%) and aadA (58.0%) for aminoglycosides, blaTEM (54.0%) for beta-lactams, qnrS (24.0%) for quinolones, tetD (66.0%) for tetracyclines, and vanB (60.0%) for glycopeptides were mainly amplified by PCR assays.
CONCLUSIONS: Vibrio genus is involved in the antibiotic resistance phenomenon diffusion in the aquatic environments, as demonstrated by the harboring of many genetic determinants representing a kind of genetic \"dark world\".
摘要：
目的：这项研究调查了在意大利中部亚得里亚海养殖供人类食用的Mytilusgalloprovincialis中鉴定出的弧菌菌株的表型和基因型耐药性。
结果：本研究涉及总共475种贻贝（Mytilusgalloprovincialis），和培养依赖的微生物学方法允许鉴定总共50个弧菌菌株，这些菌株经过抗生素敏感性测试，然后进行遗传决定子检测。抗生物图显示对氨苄青霉素的耐药性（36.0％），阿莫西林-克拉维酸（30.0％），庆大霉素（14.0%），和亚胺培南（18.0%）。生物分子测定扩增了总共264个由易感和抗性弧菌物种携带的抗生素抗性基因。在抗性基因中，氨基糖苷类的aacC2(62.0%)和aadA(58.0%)，blaTEM（54.0%）的β-内酰胺，喹诺酮类药物的qnrS（24.0%），四环素的tetD(66.0%)，糖肽的vanB（60.0％）主要通过PCR法扩增。
结论：弧菌属参与了水生环境中抗生素耐药性的扩散，正如许多代表一种遗传“黑暗世界”的遗传决定因素所证明的那样。
结果：本研究涉及总共475种贻贝（Mytilusgalloprovincialis），和培养依赖的微生物学方法允许鉴定总共50个弧菌菌株，这些菌株经过抗生素敏感性测试，然后进行遗传决定子检测。抗生物图显示对氨苄青霉素的耐药性（36.0％），阿莫西林-克拉维酸（30.0％），庆大霉素（14.0%），和亚胺培南（18.0%）。生物分子测定扩增了总共264个由易感和抗性弧菌物种携带的抗生素抗性基因。在抗性基因中，氨基糖苷类的aacC2(62.0%)和aadA(58.0%)，blaTEM（54.0%）的β-内酰胺，喹诺酮类药物的qnrS（24.0%），四环素的tetD(66.0%)，糖肽的vanB（60.0％）主要通过PCR法扩增。
结论：弧菌属参与了水生环境中抗生素耐药性的扩散，正如许多代表一种遗传“黑暗世界”的遗传决定因素所证明的那样。
