Abstract：
In this study, we investigated the diagnostic value of opsonic activity against Acinetobacter baumannii in Ventilator-Associated Pneumonia (VAP) among 50 patients, compared to 102 negative and positive controls. Out of the 50 patients, only 33 (66 %) were diagnosed with VAP using the Clinical Pulmonary Infection Score (CPIS). The opsonic activity assay demonstrated three key findings: (i) 95 % sensitivity and 91.7 % specificity, with a Receiver Operating Characteristic (ROC) area of 0.976 for distinguishing A. baumannii culture positives from negatives; (ii) 95 % sensitivity and 78.7 % specificity, with a 0.915 ROC area, in differentiating VAP/blood culture positive patients from colonized/negative groups; (iii) An ROC area of 0.553 for VAP and colonization, as identified by CPIS alone, indicating an indeterminate threshold. These results highlight that CPIS, microbiological, and clinical evaluations were not correlated, suggesting that opsonic activity against A. baumannii could be a potential VAP diagnostic tool, with the need for large-scale validations.
摘要：
在这项研究中,我们调查了50例患者对呼吸机相关性肺炎（VAP）中鲍曼不动杆菌的调理活性的诊断价值，与102个阴性和阳性对照相比。在50名患者中，只有33例(66%)使用临床肺部感染评分(CPIS)诊断为VAP.调理活性测定显示了三个关键发现：（i）95％的灵敏度和91.7％的特异性，接收器工作特征（ROC）面积为0.976，用于区分鲍曼不动杆菌培养阳性和阴性；（ii）95％的灵敏度和78.7％的特异性，ROC为0.915，区分VAP/血培养阳性患者与定植/阴性组；（iii）VAP和定植的ROC面积为0.553，仅由CPIS确定，指示不确定的阈值。这些结果突显了CPIS，微生物,和临床评估没有相关性，这表明对鲍曼不动杆菌的调理活性可能是一种潜在的VAP诊断工具，需要进行大规模验证。
