RESULTS: LC-MS/MS technology is used to investigate the peptide distribution in both samples. The immunoreactivity of two PHF-Ws in 27 serum samples from 50 Chinese infants (02 years) with CMA is analyzed. The results demonstrate that even with a similar a degree of hydrolysis (DH), primary protein sources, peptides with molecular weights <5 kDa, and differences in the number of residual allergenic epitopes in the hydrolyzed peptide segments can lead to varying immune responses.
CONCLUSIONS: The two PHF-Ws have notably high intolerance rates, exceeding 10% among infants with CMA. Therefore, suggesting that PHF-Ws may not be suitable for infants and children with CMA in China.
结果:LC-MS/MS技术用于研究两个样品中的肽分布。分析了来自50名患有CMA的中国婴儿(02岁)的27份血清样品中两种PHF-Ws的免疫反应性。结果表明,即使具有相似的水解度(DH),主要蛋白质来源,分子量<5kDa的肽,水解肽段中残留变应原性表位数量的差异可导致不同的免疫应答。
结论:两种PHF-W具有明显的高不耐受率,CMA婴儿超过10%。因此,提示PHF-Ws可能不适合中国CMA婴幼儿。