Abstract：
METHODS: In cases where breast milk is unavailable or inadequate, hydrolyzed infant formula is recommended as the primary alternative. The aim of this study is to assess and compare the allergenicity of two partially hydrolyzed whey-based formulas (PHF-Ws) using serum samples from patients with cow\'s milk allergy (CMA).
RESULTS: LC-MS/MS technology is used to investigate the peptide distribution in both samples. The immunoreactivity of two PHF-Ws in 27 serum samples from 50 Chinese infants (02 years) with CMA is analyzed. The results demonstrate that even with a similar a degree of hydrolysis (DH), primary protein sources, peptides with molecular weights <5 kDa, and differences in the number of residual allergenic epitopes in the hydrolyzed peptide segments can lead to varying immune responses.
CONCLUSIONS: The two PHF-Ws have notably high intolerance rates, exceeding 10% among infants with CMA. Therefore, suggesting that PHF-Ws may not be suitable for infants and children with CMA in China.
RESULTS: LC-MS/MS technology is used to investigate the peptide distribution in both samples. The immunoreactivity of two PHF-Ws in 27 serum samples from 50 Chinese infants (02 years) with CMA is analyzed. The results demonstrate that even with a similar a degree of hydrolysis (DH), primary protein sources, peptides with molecular weights <5 kDa, and differences in the number of residual allergenic epitopes in the hydrolyzed peptide segments can lead to varying immune responses.
CONCLUSIONS: The two PHF-Ws have notably high intolerance rates, exceeding 10% among infants with CMA. Therefore, suggesting that PHF-Ws may not be suitable for infants and children with CMA in China.
摘要：
方法：在母乳不可用或不足的情况下，水解婴儿配方奶粉被推荐为主要替代品。这项研究的目的是使用来自牛奶过敏（CMA）患者的血清样本评估和比较两种基于部分水解乳清的配方（PHF-Ws）的过敏原性。
结果：LC-MS/MS技术用于研究两个样品中的肽分布。分析了来自50名患有CMA的中国婴儿（02岁）的27份血清样品中两种PHF-Ws的免疫反应性。结果表明，即使具有相似的水解度（DH），主要蛋白质来源，分子量<5kDa的肽，水解肽段中残留变应原性表位数量的差异可导致不同的免疫应答。
结论：两种PHF-W具有明显的高不耐受率，CMA婴儿超过10%。因此,提示PHF-Ws可能不适合中国CMA婴幼儿。
结果：LC-MS/MS技术用于研究两个样品中的肽分布。分析了来自50名患有CMA的中国婴儿（02岁）的27份血清样品中两种PHF-Ws的免疫反应性。结果表明，即使具有相似的水解度（DH），主要蛋白质来源，分子量<5kDa的肽，水解肽段中残留变应原性表位数量的差异可导致不同的免疫应答。
结论：两种PHF-W具有明显的高不耐受率，CMA婴儿超过10%。因此,提示PHF-Ws可能不适合中国CMA婴幼儿。
