Abstract：
Ribosome biogenesis, involving processing/assembly of rRNAs and r-proteins is a vital process. In Saccharomyces cerevisiae mitochondria, ribosomal small subunit comprises 15S rRNA (15S). While the 15S 5\'-end processing uses Ccm1p and Pet127p, the mechanisms of the 3\'-end processing remain unclear. We reveal involvement of Rmd9p in safeguarding/processing 15S 3\'-end. Rmd9p deficiency results in a cleavage at a position 183 nucleotides upstream of 15S 3\'-end, and in the loss of the 3\'-minor domain. Rmd9p binds to the sequences in the 3\'-end region of 15S, and a genetic interaction between rmd9 and dss1 indicates that Rmd9p regulates/limits mtEXO activity during the 3\'-end spacer processing.
摘要：
核糖体生物发生，涉及rRNA和r蛋白的加工/组装是一个至关重要的过程。在酿酒酵母线粒体中，核糖体小亚基包含15SrRNA(15S)。虽然15S5'端处理使用Ccm1p和Pet127p，3'端处理的机制尚不清楚。我们揭示了Rmd9p参与保护/处理15S3'-end。Rmd9p缺陷导致在15S3'-末端上游183个核苷酸的位置切割，并在失去3\'-次要域名的情况下。Rmd9p结合15S前体中的3'端间隔区序列，rmd9和dss1之间的遗传相互作用表明Rmd9p在3'端间隔区加工过程中调节/限制mtEXO活性。
