Abstract：
Yellow fever (YF) is one of the most acute viral hemorrhagic diseases of the 18th and 19th centuries, which continues to cause severe morbidity and mortality in Africa. After 21 years of no reported cases of yellow fever in Nigeria, till 2017 where a case was confirmed in Kwara State, also in November 2018,WHO was informed of a cluster of suspected yellow fever cases and deaths in Edo state, Nigeria. The study was among all age group attending health centres in Benin City, Edo state. A total of 280 blood samples were collected from consented febrile patients and were screened for antibodies to Zika virus using rapid diagnostic test (RDT) kits. Blood samples positive to Zika virus (IgM/IgG RDT), were subjected to molecular characterization. Using the flavividae family primers, six (6) samples where confirmed positive by Hemi-nested reverse transcription PCR (hnRT-PCR) sequencing. Nucleotide sequence blast revealed the sequenceswere similar to Yellow fever virus strains. Phylogenetic analysis revealed that the yellow fever virus sequences are closely related to the African strains. Despite the safe and effective yellow fever vaccine, yellow fever virus is seen to be in circulation, hence the need for continues mass vaccination.
摘要：
黄热病（YF）是18和19世纪最急性的病毒性出血性疾病之一，继续在非洲造成严重的发病率和死亡率。在尼日利亚21年没有报告黄热病病例之后，直到2017年在Kwara州确认了一个病例，也是在2018年11月，世卫组织获悉江户州出现了一批疑似黄热病病例和死亡病例，尼日利亚。这项研究是在贝宁市卫生中心就诊的所有年龄组中进行的，江户州。从同意的发热患者中收集了总共280份血液样品，并使用快速诊断测试（RDT）试剂盒筛选了针对寨卡病毒的抗体。寨卡病毒（IgM/IgGRDT）阳性的血液样本，进行了分子表征。使用黄科科引物，通过半巢式逆转录PCR(hnRT-PCR)测序证实为阳性的六(6)份样品。核苷酸序列爆炸显示序列与黄热病病毒株相似。系统发育分析表明，黄热病病毒序列与非洲毒株密切相关。尽管黄热病疫苗安全有效，黄热病病毒被认为在循环中，因此需要继续进行大规模疫苗接种。
