Abstract:
Vibrio parahaemolyticus is a major foodborne pathogen causing serious illnesses. In this study, a new lytic bacteriophage SSJ01 that infects V. parahaemolyticus was isolated and characterized. It had a short non-contractile tail and belonged to the Caudoviricetes class. It rapidly adsorbed onto host cells, exhibited a short latent period, and has a large burst size. It showed lytic activities under a broad range of temperature (- 18 to 60 °C), pH (5 to 11), and salinity (0 to 6%). It contained 35 open reading frames with a G + C content of 49.16% without toxic or lysogen-forming genes. The MOI of 105 phage-treated group in vitro reduced the target cells up to 3.49-log CFU/mL at 6 °C and 3.47-log CFU/mL at 25 °C, respectively. In aquatic environments (6 and 25 °C), bactericidal activities showed a significant decrease within 2 h. Therefore, the bacteriophage SSJ01 has potential as a biocontrol agent to control V. parahaemolyticus in marine culture.
摘要:
副溶血性弧菌是引起严重疾病的主要食源性病原体。在这项研究中,分离并鉴定了感染副溶血性弧菌的新的裂解噬菌体SSJ01。它有一个短的无收缩尾巴,属于Caudoviricetes类。它迅速吸附在宿主细胞上,表现出短暂的潜伏期,并有一个大的突发大小。它在广泛的温度范围(-18至60°C)下显示出裂解活性,pH(5至11),和盐度(0至6%)。它包含35个开放阅读框,GC含量为49.16%,没有毒性或溶原形成基因。105个噬菌体处理组的MOI在体外将靶细胞在6°C时降低至3.49-logCFU/mL,在25°C时降低至3.47-logCFU/mL,分别。在水生环境中(6和25°C),杀菌活性在2h内显着降低。因此,噬菌体SSJ01具有作为生物防治剂在海洋培养中控制副溶血性弧菌的潜力。