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Abstract：
Vibrio parahaemolyticus is a major foodborne pathogen causing serious illnesses. In this study, a new lytic bacteriophage SSJ01 that infects V. parahaemolyticus was isolated and characterized. It had a short non-contractile tail and belonged to the Caudoviricetes class. It rapidly adsorbed onto host cells, exhibited a short latent period, and has a large burst size. It showed lytic activities under a broad range of temperature (- 18 to 60 °C), pH (5 to 11), and salinity (0 to 6%). It contained 35 open reading frames with a G + C content of 49.16% without toxic or lysogen-forming genes. The MOI of 105 phage-treated group in vitro reduced the target cells up to 3.49-log CFU/mL at 6 °C and 3.47-log CFU/mL at 25 °C, respectively. In aquatic environments (6 and 25 °C), bactericidal activities showed a significant decrease within 2 h. Therefore, the bacteriophage SSJ01 has potential as a biocontrol agent to control V. parahaemolyticus in marine culture.
摘要：
副溶血性弧菌是引起严重疾病的主要食源性病原体。在这项研究中,分离并鉴定了感染副溶血性弧菌的新的裂解噬菌体SSJ01。它有一个短的无收缩尾巴，属于Caudoviricetes类。它迅速吸附在宿主细胞上，表现出短暂的潜伏期，并有一个大的突发大小。它在广泛的温度范围（-18至60°C）下显示出裂解活性，pH（5至11），和盐度（0至6％）。它包含35个开放阅读框，GC含量为49.16％，没有毒性或溶原形成基因。105个噬菌体处理组的MOI在体外将靶细胞在6°C时降低至3.49-logCFU/mL，在25°C时降低至3.47-logCFU/mL，分别。在水生环境中（6和25°C），杀菌活性在2h内显着降低。因此，噬菌体SSJ01具有作为生物防治剂在海洋培养中控制副溶血性弧菌的潜力。
