UNASSIGNED: We investigated whether onion bulb extract (OBE) can (1) reverse established asthma phenotype (therapeutic treatment) and/or (2) prevent the development of the asthma phenotype, if given before the immunization process (preventative treatment).
UNASSIGNED: Six groups of male Balb/c mice were established for the therapeutic (21 days) and five groups for the preventative (19 days) treatment protocols; including PBS and house dust mite (HDM)-challenged mice treated with vehicle or OBE (30, 60, and 100 mg/kg/i.p.). Airways inflammation was determined using cytology, histology, immunofluorescence, Western blot, and serum IgE.
UNASSIGNED: Therapeutic (60 mg/kg/i.p.) and preventative (100 mg/kg/i.p.) OBE treatment resulted in down-regulation of HDM-induced airway cellular influx, histopathological changes and the increase in expression of pro-inflammatory signaling pathway EGFR, ERK1/2, AKT, pro-inflammatory cytokines and serum IgE.
UNASSIGNED: Our data show that OBE is an effective anti-inflammatory agent with both therapeutic and preventative anti-asthma effects. These findings imply that onion/OBE may be used as an adjunct therapeutic agent in established asthma and/or to prevent development of allergic asthma. However, further studies to identify the active constituents, and demonstrate proof-of-concept in humans are needed.
■我们研究了洋葱鳞茎提取物(OBE)是否可以(1)逆转已确定的哮喘表型(治疗性治疗)和/或(2)预防哮喘表型的发展,如果在免疫过程之前给予(预防性治疗)。
■建立六组雄性Balb/c小鼠用于治疗性(21天),并建立五组用于预防性(19天)治疗方案;包括用载体或OBE(30、60和100mg/kg/i.p.)治疗的PBS和屋尘螨(HDM)攻击的小鼠。使用细胞学检查确定气道炎症,组织学,免疫荧光,蛋白质印迹,和血清IgE。
■治疗性(60mg/kg/i.p.)和预防性(100mg/kg/i.p.)OBE治疗导致HDM诱导的气道细胞内流下调,组织病理学改变和促炎信号通路EGFR的表达增加,ERK1/2,AKT,促炎细胞因子和血清IgE。
■我们的数据表明OBE是一种有效的抗炎药,具有治疗和预防哮喘作用。这些发现暗示洋葱/OBE可用作已确定的哮喘的辅助治疗剂和/或预防过敏性哮喘的发展。然而,进一步的研究,以确定活性成分,并证明人类需要概念证明。