PDF(Pubmed)
Abstract：
UNASSIGNED: Asthma presents a global health challenge. The main pharmacotherapy is synthetic chemicals and biological-based drugs that are costly, and have significant side effects. In contrast, use of natural products, such as onion (Allium cepa L., Amaryllidaceae) in the treatment of airway diseases has increased world-wide because of their perceived efficacy and little safety concerns. However, their pharmacological actions remain largely uncharacterized.
UNASSIGNED: We investigated whether onion bulb extract (OBE) can (1) reverse established asthma phenotype (therapeutic treatment) and/or (2) prevent the development of the asthma phenotype, if given before the immunization process (preventative treatment).
UNASSIGNED: Six groups of male Balb/c mice were established for the therapeutic (21 days) and five groups for the preventative (19 days) treatment protocols; including PBS and house dust mite (HDM)-challenged mice treated with vehicle or OBE (30, 60, and 100 mg/kg/i.p.). Airways inflammation was determined using cytology, histology, immunofluorescence, Western blot, and serum IgE.
UNASSIGNED: Therapeutic (60 mg/kg/i.p.) and preventative (100 mg/kg/i.p.) OBE treatment resulted in down-regulation of HDM-induced airway cellular influx, histopathological changes and the increase in expression of pro-inflammatory signaling pathway EGFR, ERK1/2, AKT, pro-inflammatory cytokines and serum IgE.
UNASSIGNED: Our data show that OBE is an effective anti-inflammatory agent with both therapeutic and preventative anti-asthma effects. These findings imply that onion/OBE may be used as an adjunct therapeutic agent in established asthma and/or to prevent development of allergic asthma. However, further studies to identify the active constituents, and demonstrate proof-of-concept in humans are needed.
UNASSIGNED: We investigated whether onion bulb extract (OBE) can (1) reverse established asthma phenotype (therapeutic treatment) and/or (2) prevent the development of the asthma phenotype, if given before the immunization process (preventative treatment).
UNASSIGNED: Six groups of male Balb/c mice were established for the therapeutic (21 days) and five groups for the preventative (19 days) treatment protocols; including PBS and house dust mite (HDM)-challenged mice treated with vehicle or OBE (30, 60, and 100 mg/kg/i.p.). Airways inflammation was determined using cytology, histology, immunofluorescence, Western blot, and serum IgE.
UNASSIGNED: Therapeutic (60 mg/kg/i.p.) and preventative (100 mg/kg/i.p.) OBE treatment resulted in down-regulation of HDM-induced airway cellular influx, histopathological changes and the increase in expression of pro-inflammatory signaling pathway EGFR, ERK1/2, AKT, pro-inflammatory cytokines and serum IgE.
UNASSIGNED: Our data show that OBE is an effective anti-inflammatory agent with both therapeutic and preventative anti-asthma effects. These findings imply that onion/OBE may be used as an adjunct therapeutic agent in established asthma and/or to prevent development of allergic asthma. However, further studies to identify the active constituents, and demonstrate proof-of-concept in humans are needed.
摘要：
■哮喘是一个全球性的健康挑战。主要的药物治疗是合成化学品和生物药物，成本很高，并有明显的副作用。相比之下,使用天然产品，如洋葱(洋葱，Amaryllic科）在气道疾病的治疗中由于其感知的功效和很少的安全性问题而在世界范围内有所增加。然而,它们的药理作用在很大程度上仍未表征。
■我们研究了洋葱鳞茎提取物（OBE）是否可以（1）逆转已确定的哮喘表型（治疗性治疗）和/或（2）预防哮喘表型的发展，如果在免疫过程之前给予（预防性治疗）。
■建立六组雄性Balb/c小鼠用于治疗性(21天),并建立五组用于预防性(19天)治疗方案;包括用载体或OBE(30、60和100mg/kg/i.p.)治疗的PBS和屋尘螨(HDM)攻击的小鼠。使用细胞学检查确定气道炎症，组织学,免疫荧光，蛋白质印迹,和血清IgE。
■治疗性（60mg/kg/i.p.）和预防性（100mg/kg/i.p.）OBE治疗导致HDM诱导的气道细胞内流下调，组织病理学改变和促炎信号通路EGFR的表达增加，ERK1/2,AKT,促炎细胞因子和血清IgE。
■我们的数据表明OBE是一种有效的抗炎药，具有治疗和预防哮喘作用。这些发现暗示洋葱/OBE可用作已确定的哮喘的辅助治疗剂和/或预防过敏性哮喘的发展。然而,进一步的研究，以确定活性成分，并证明人类需要概念证明。
■我们研究了洋葱鳞茎提取物（OBE）是否可以（1）逆转已确定的哮喘表型（治疗性治疗）和/或（2）预防哮喘表型的发展，如果在免疫过程之前给予（预防性治疗）。
■建立六组雄性Balb/c小鼠用于治疗性(21天),并建立五组用于预防性(19天)治疗方案;包括用载体或OBE(30、60和100mg/kg/i.p.)治疗的PBS和屋尘螨(HDM)攻击的小鼠。使用细胞学检查确定气道炎症，组织学,免疫荧光，蛋白质印迹,和血清IgE。
■治疗性（60mg/kg/i.p.）和预防性（100mg/kg/i.p.）OBE治疗导致HDM诱导的气道细胞内流下调，组织病理学改变和促炎信号通路EGFR的表达增加，ERK1/2,AKT,促炎细胞因子和血清IgE。
■我们的数据表明OBE是一种有效的抗炎药，具有治疗和预防哮喘作用。这些发现暗示洋葱/OBE可用作已确定的哮喘的辅助治疗剂和/或预防过敏性哮喘的发展。然而,进一步的研究，以确定活性成分，并证明人类需要概念证明。
