Abstract：
BACKGROUND: The emergence of DNA nanotechnology has enabled the systematic design of diverse bionic dissipative behaviors under the precise control of nucleic acid nanodevices. Nevertheless, when compared to the dissipation observed in robust living systems, it is highly desirable to enhance the anti-interference for artificial DNA dissipation to withstand perturbations and facilitate repairs within the complex biological environments.
RESULTS: In this study, we introduce strategically designed \"trash cans\" to facilitate kinetic control over interferences, transforming the stochastic binding of individual components within a homogeneous solution into a competitive binding process. This approach effectively eliminates incorrect binding and the accumulation of systemic interferences while ensuring a consistent pattern of energy fluctuation from response to silence. Remarkably, even in the presence of numerous interferences differing by only one base, we successfully achieve complete system reset through multiple cycles, effectively restoring the energy level to a minimum.
CONCLUSIONS: The system was able to operate stably without any adverse effect under conditions of irregular interference, high-abundance interference, and even multiplex interferences including DNA and RNA crosstalk. This work not only provides an effective paradigm for constructing robust DNA dissipation systems but also greatly broadens the potential of DNA dissipation for applications in high-precision molecular recognition and complex biological reaction networks.
RESULTS: In this study, we introduce strategically designed \"trash cans\" to facilitate kinetic control over interferences, transforming the stochastic binding of individual components within a homogeneous solution into a competitive binding process. This approach effectively eliminates incorrect binding and the accumulation of systemic interferences while ensuring a consistent pattern of energy fluctuation from response to silence. Remarkably, even in the presence of numerous interferences differing by only one base, we successfully achieve complete system reset through multiple cycles, effectively restoring the energy level to a minimum.
CONCLUSIONS: The system was able to operate stably without any adverse effect under conditions of irregular interference, high-abundance interference, and even multiplex interferences including DNA and RNA crosstalk. This work not only provides an effective paradigm for constructing robust DNA dissipation systems but also greatly broadens the potential of DNA dissipation for applications in high-precision molecular recognition and complex biological reaction networks.
摘要：
背景：DNA纳米技术的出现使在核酸纳米器件的精确控制下对多种仿生耗散行为进行了系统设计。然而，与在健壮的生命系统中观察到的耗散相比，高度期望增强人工DNA耗散的抗干扰以承受扰动并促进复杂生物环境内的修复。
结果：在这项研究中，我们引入了战略性设计的“垃圾桶”，以促进对干扰的动力学控制，将均匀解决方案中单个组件的随机绑定转换为竞争性绑定过程。这种方法有效地消除了不正确的结合和系统干扰的积累，同时确保了从响应到沉默的能量波动的一致模式。值得注意的是,即使存在只有一个基数的众多干扰，我们通过多个周期成功地实现了完整的系统复位，有效地将能量水平恢复到最低。
结论：系统能够在不规则干扰条件下稳定运行，没有任何不利影响，高丰度干扰，甚至包括DNA和RNA串扰在内的多重干扰。这项工作不仅为构建强大的DNA耗散系统提供了有效的范例，而且极大地拓宽了DNA耗散在高精度分子识别和复杂生物反应网络中的应用潜力。
结果：在这项研究中，我们引入了战略性设计的“垃圾桶”，以促进对干扰的动力学控制，将均匀解决方案中单个组件的随机绑定转换为竞争性绑定过程。这种方法有效地消除了不正确的结合和系统干扰的积累，同时确保了从响应到沉默的能量波动的一致模式。值得注意的是,即使存在只有一个基数的众多干扰，我们通过多个周期成功地实现了完整的系统复位，有效地将能量水平恢复到最低。
结论：系统能够在不规则干扰条件下稳定运行，没有任何不利影响，高丰度干扰，甚至包括DNA和RNA串扰在内的多重干扰。这项工作不仅为构建强大的DNA耗散系统提供了有效的范例，而且极大地拓宽了DNA耗散在高精度分子识别和复杂生物反应网络中的应用潜力。
