Abstract：
CA125 (carbohydrate antigen 125) is an important biomarker of ovarian cancer, so developing effective method for its detection is of great significance. In the present work, a novel sandwich-like electrochemical immunosensor (STEM) of CA125 was constructed by preparing nanoribbon-like Ti3C2Tx MXenes (Ti3C2TxNR) to immobilize primary antibody (PAb) of CA125 and UIO-66-NH2 MOFs structure to immobilize second antibody (SAb) and electroactive toluidine blue (Tb) probe. In this designed STEM assay, the as-prepared Ti3C2TxNR nanohybrid offers the advantages in large surface area and conductivity as carrier, and UIO-66-NH2 provided an ideal platform to accommodate SAb and a large number of Tb molecules as signal amplifier. In the presence of CA125, the peak currents of Tb from the formed STEM structure increase with the increase of CA125 level. After optimizing the related control conditions, a wide linear range (0.2-150.0 U mL-1) and a very low detection limit (0.05 U mL-1) of CA125 were achieved. It\'s thus expected the developed STEM strategy has important applications for the detection of CA125.
摘要：
CA125(糖类抗原125)是卵巢癌的重要生物标志物,因此开发有效的检测方法具有重要意义。在目前的工作中,通过制备纳米带样Ti3C2TxMXenes（Ti3C2TxNR）来固定CA125和UIO-66-NH2MOFs结构的第一抗体（PAb），以固定第二抗体（SAb）和电活性甲苯胺蓝（Tb）探针，构建了一种新型的CA125夹心样电化学免疫传感器（STEM）。在这个设计的STEM分析中，制备的Ti3C2TxNR纳米杂化物具有大表面积和作为载体的导电性等优点，和UIO-66-NH2提供了一个理想的平台来容纳SAb和大量的Tb分子作为信号放大器。在存在CA125的情况下,来自所形成的STEM结构的Tb的峰值电流随着CA125水平的增加而增加。优化相关控制条件后,CA125的线性范围（0.2-150.0UmL-1）和检测限（0.05UmL-1）很低。因此，预计开发的STEM策略对于CA125的检测具有重要的应用。
