Abstract：
This study evaluates the repurposing of expired isopropanol (IPA) COVID-19 disinfectant (64% w/w) to pretreat algal biomass for enhancing methane (CH4) yield. The impact of harvesting methods (centrifugation and polymer flocculation) and microwave pretreatment on CH4 production from Scenedesmus sp. microalgal biomass were also investigated. Results show minimal impact of harvesting methods on the CH4 yield, with wet centrifuged and polymer-harvested biomass exhibiting comparable and low CH4 production at 66 and 74 L/kgvolatile solid, respectively. However, microalgae drying significantly increased CH4 yield compared to wet biomass, attributed to cell shrinkage and enhanced digestibility. Consequently, microwave and IPA pretreatment significantly enhanced CH4 production when applied to dried microalgae, yielding a 135% and 212% increase, respectively, compared to non-pretreated wet biomass. These findings underscore the advantage of using dried Scenedesmus sp. over wet biomass and highlight the synergistic effect of combining oven drying with IPA treatment to boost CH4 production whilst reducing COVID-19 waste.
摘要：
这项研究评估了过期异丙醇（IPA）COVID-19消毒剂（64％w/w）的再利用，以预处理藻类生物质以提高甲烷（CH4）产量。收获方法（离心和聚合物絮凝）和微波预处理对Scenedesmussp生产CH4的影响。还研究了微藻生物量。结果表明，收获方法对CH4产量的影响最小，湿离心和聚合物收获的生物质在66和74升/千克挥发性固体下表现出相当且低的CH4产量，分别。然而,与湿生物质相比，微藻干燥显着提高了CH4产量，归因于细胞收缩和消化率增强。因此,微波和IPA预处理显着提高CH4的产量，当应用于干燥的微藻，产生135%和212%的增长，分别,与未预处理的湿生物质相比。这些发现强调了使用干燥的Scenedesmussp的优势。并突出了烘箱干燥与IPA处理相结合的协同效应，以提高CH4产量，同时减少COVID-19废物的关注。
