Abstract：
Identification of methods for the standardized assessment of bacterial pathogens and antimicrobial resistance (AMR) in environmental water can improve the quality of monitoring and data collected, support global surveillance efforts, and enhance the understanding of environmental water sources. We conducted a systematic review to assemble and synthesize available literature that identified methods for assessment of prevalence and abundance of bacterial fecal indicators and pathogens in water for the purposes of monitoring bacterial pathogens and AMR. After screening for quality, 175 unique publications were identified from 15 databases, and data were extracted for analysis. This review identifies the most common and robust methods, and media used to isolate target organisms from surface water sources, summarizes methodological trends, and recognizes knowledge gaps. The information presented in this review will be useful when establishing standardized methods for monitoring bacterial pathogens and AMR in water in the United States and globally.
摘要：
确定环境水中细菌病原体和抗菌素耐药性（AMR）的标准化评估方法可以提高监测和收集数据的质量，支持全球监测工作，加强对环境水源的认识。我们进行了系统综述，以收集和综合现有文献，确定了评估水中细菌粪便指标和病原体的患病率和丰度的方法，以监测细菌病原体和AMR。经过质量筛选，从15个数据库中确定了175种独特的出版物，并提取数据进行分析。这篇综述确定了最常见和最可靠的方法，和用于从地表水源分离目标生物的培养基，总结了方法论趋势，并认识到知识差距。在美国和全球建立监测水中细菌病原体和AMR的标准化方法时，本综述中提供的信息将很有用。
