Abstract：
BACKGROUND: Sicklepod [Cassia obtusifolia L. syn Senna obtusifolia (L.) H.S. Irwin & Barneby, Fabaceae] sprouts are promising ingredients with health-promoting benefits. Notwithstanding, the pharmacologically active compounds in sicklepod sprouts have not been studied or analysed in detail.
OBJECTIVE: This study aimed to isolate and structurally identify phytochemicals showing α-glucosidase inhibitory activity in sicklepod sprouts and simultaneously quantify the compounds in the sprouts to determine the optimal cultivation method and germination time to maximise active compounds.
METHODS: A simultaneous high-performance liquid chromatography-ultraviolet (HPLC-UV) method with high sensitivity and accuracy was developed and used to analyse time-dependent changes in anthraquinone content during sicklepod germination.
RESULTS: Thirteen anthraquinones were isolated and identified, of which six-chrysoobtusin, emodin, 1-O-methyl-2-methoxychrysophanol, 7-O-methylobtusin, chrysophanol, and physcion-showed moderate α-glucosidase inhibitory activity. The maximum content of anthraquinones in a sprout was observed on Day 5 under both light and dark conditions.
CONCLUSIONS: The findings of this study revealed that sicklepod sprouts which are promising functional food materials contain a variety of anthraquinones.
OBJECTIVE: This study aimed to isolate and structurally identify phytochemicals showing α-glucosidase inhibitory activity in sicklepod sprouts and simultaneously quantify the compounds in the sprouts to determine the optimal cultivation method and germination time to maximise active compounds.
METHODS: A simultaneous high-performance liquid chromatography-ultraviolet (HPLC-UV) method with high sensitivity and accuracy was developed and used to analyse time-dependent changes in anthraquinone content during sicklepod germination.
RESULTS: Thirteen anthraquinones were isolated and identified, of which six-chrysoobtusin, emodin, 1-O-methyl-2-methoxychrysophanol, 7-O-methylobtusin, chrysophanol, and physcion-showed moderate α-glucosidase inhibitory activity. The maximum content of anthraquinones in a sprout was observed on Day 5 under both light and dark conditions.
CONCLUSIONS: The findings of this study revealed that sicklepod sprouts which are promising functional food materials contain a variety of anthraquinones.
摘要：
背景：Sicklepod[决明子L.synSennaobtusifolia（L.)H.S.Irwin&Barneby,豆科]豆芽是具有促进健康益处的有前途的成分。尽管如此,镰刀豆芽中的药理活性化合物尚未得到详细研究或分析。
目的：本研究旨在分离和结构鉴定在镰刀菌豆芽中显示α-葡萄糖苷酶抑制活性的植物化学物质，并同时定量豆芽中的化合物，以确定最佳的培养方法和发芽时间，以最大化活性化合物。
方法：建立了一种同时具有高灵敏度和准确性的高效液相色谱-紫外（HPLC-UV）方法，用于分析镰刀菌萌发过程中蒽醌含量随时间的变化。
结果：分离并鉴定了13种蒽醌，其中6-chrysoobtusin，大黄素,1-O-甲基-2-甲氧基大黄酚，7-O-甲基甘油,大黄酚,和physcion-显示中等的α-葡萄糖苷酶抑制活性。在第5天，在光照和黑暗条件下观察到芽中蒽醌的最大含量。
结论：这项研究的发现表明，作为有前途的功能性食品材料，镰状豆芽含有多种蒽醌。
目的：本研究旨在分离和结构鉴定在镰刀菌豆芽中显示α-葡萄糖苷酶抑制活性的植物化学物质，并同时定量豆芽中的化合物，以确定最佳的培养方法和发芽时间，以最大化活性化合物。
方法：建立了一种同时具有高灵敏度和准确性的高效液相色谱-紫外（HPLC-UV）方法，用于分析镰刀菌萌发过程中蒽醌含量随时间的变化。
结果：分离并鉴定了13种蒽醌，其中6-chrysoobtusin，大黄素,1-O-甲基-2-甲氧基大黄酚，7-O-甲基甘油,大黄酚,和physcion-显示中等的α-葡萄糖苷酶抑制活性。在第5天，在光照和黑暗条件下观察到芽中蒽醌的最大含量。
结论：这项研究的发现表明，作为有前途的功能性食品材料，镰状豆芽含有多种蒽醌。
