%0 Journal Article
%T Simultaneous high-performance liquid chromatography analysis of anthraquinones in sicklepod sprouts with α-glucosidase inhibitory activity.
%A Muraoka T
%A Imahori D
%A Miyagi R
%A Shinohara N
%A Tanaka H
%J Phytochem Anal
%V 35
%N 5
%D 2024 Jul 4
%M 38572825
%F 3.024
%R 10.1002/pca.3353
%X <B>BACKGROUND: </B>Sicklepod [Cassia obtusifolia L. syn Senna obtusifolia (L.) H.S. Irwin & Barneby, Fabaceae] sprouts are promising ingredients with health-promoting benefits. Notwithstanding, the pharmacologically active compounds in sicklepod sprouts have not been studied or analysed in detail.<BR><B>OBJECTIVE: </B>This study aimed to isolate and structurally identify phytochemicals showing α-glucosidase inhibitory activity in sicklepod sprouts and simultaneously quantify the compounds in the sprouts to determine the optimal cultivation method and germination time to maximise active compounds.<BR><B>METHODS: </B>A simultaneous high-performance liquid chromatography-ultraviolet (HPLC-UV) method with high sensitivity and accuracy was developed and used to analyse time-dependent changes in anthraquinone content during sicklepod germination.<BR><B>RESULTS: </B>Thirteen anthraquinones were isolated and identified, of which six-chrysoobtusin, emodin, 1-O-methyl-2-methoxychrysophanol, 7-O-methylobtusin, chrysophanol, and physcion-showed moderate α-glucosidase inhibitory activity. The maximum content of anthraquinones in a sprout was observed on Day 5 under both light and dark conditions.<BR><B>CONCLUSIONS: </B>The findings of this study revealed that sicklepod sprouts which are promising functional food materials contain a variety of anthraquinones.