Abstract：
The prognosis of autoimmune thyroid diseases (AITDs), including Hashimoto\'s disease (HD) and Graves\' disease (GD), is difficult to predict. DNA methylation regulates gene expression of immune mediating factors. Interleukin (IL)-10 is a Th2 cytokine that downregulates inflammatory cytokines produced by Th1 cells. To clarify the role of methylation of the IL10 gene in the prognosis of AITD, we evaluated the methylation levels of two CpG sites in the IL10 promoter using pyrosequencing. The methylation levels of the -185 CpG site of the IL10 gene were related to age and GD intractability in GD patients. Furthermore, the C carrier of the IL10-592 A/C polymorphism was related to low methylation levels of the -185 CpG site. The methylation levels of the IL10-185 CpG site of the IL10 gene were related to the intractability of GD and were lower in individuals with the C allele of the IL10-592 A/C polymorphism.
摘要：
自身免疫性甲状腺疾病（AITDs）的预后，包括桥本病（HD）和格雷夫斯病（GD），很难预测。DNA甲基化调节免疫介导因子的基因表达。白细胞介素(IL)-10是下调由Th1细胞产生的炎性细胞因子的Th2细胞因子。阐明IL10基因甲基化在AITD预后中的作用,我们使用焦磷酸测序评估了IL10启动子中两个CpG位点的甲基化水平.在GD患者中，IL10基因的-185CpG位点的甲基化水平与年龄和GD难处理性有关。此外，IL10-592A/C多态性的C携带者与-185CpG位点的低甲基化水平有关。IL10基因的IL10-185CpG位点的甲基化水平与GD的难处理性有关，在具有IL10-592A/C多态性的C等位基因的个体中，甲基化水平较低。
