PDF(Pubmed)
Abstract：
Desmosomes are intercellular junctions that regulate mechanical integrity in epithelia and cardiac muscle. Dynamic desmosome remodeling is essential for wound healing and development, yet the mechanisms governing junction assembly remain elusive. While we and others have shown that cadherin ectodomains are highly organized, how this ordered architecture emerges during assembly is unknown. Using fluorescence polarization microscopy, we show that desmoglein 2 (Dsg2) ectodomain order gradually increases during 8 h of assembly, coinciding with increasing adhesive strength. In a scratch wound assay, we observed a similar increase in order in desmosomes assembling at the leading edge of migratory cells. Together, our findings indicate that cadherin organization is a hallmark of desmosome maturity and may play a role in conferring adhesive strength.
摘要：
桥粒是调节上皮和心肌机械完整性的细胞间连接。动态桥粒重塑对伤口愈合和发育至关重要，然而，控制连接组装的机制仍然难以捉摸。虽然我们和其他人已经表明钙粘蛋白胞外域是高度组织化的，这个有序的架构如何在组装过程中出现是未知的。使用荧光偏振显微镜，我们发现，桥粒2（Dsg2）胞外域顺序在组装的8小时内逐渐增加，与粘合强度增加相吻合。在划痕伤口分析中，我们观察到迁移细胞前缘的桥粒组装顺序有类似的增加。一起,我们的发现表明，钙黏着蛋白的组织是桥粒成熟的标志，可能在赋予粘附强度中起作用。
