Abstract：
CONCLUSIONS: EgMADS3, a pivotal transcription factor, positively regulates MCFA accumulation via binding to the EgLPAAT promoter, advancing lipid content in mesocarp of oil palm. Lipids function as the structural components of cell membranes, which serve as permeable barriers to the external environment of cells. The medium-chain fatty acid in the stored lipids of plants is an important renewable energy. Most research on MCFA production in plant lipid synthesis is based on biochemical methods, and the importance of transcriptional regulation in MCFA synthesis and its incorporation into TAGs needs further research. Oil palm is the most productive oil crop in the world and has the highest productivity among the main oil crops. In this study, the MADS transcription factor (EgMADS3) in the mesocarp of oil palm was characterized. Through the VIGS-virus induced gene silencing, it was determined that the potential target gene of EgMADS3 was related to the biosynthesis of medium-chain fatty acid (MCFA). Transient transformation in protoplasts and qRT-PCR analysis showed that EgMADS3 positively regulated the expression of EgLPAAT. The results of the yeast one-hybrid assays and EMSA indicated the interaction between EgMADS3 and EgLPAAT promoter. Through genetic transformation and fatty acid analysis, it is concluded that EgMADS3 directly regulates the mid-chain fatty acid synthesis pathway of the potential target gene EgLPAAT, thus promotes the accumulation of MCFA and improves the total lipid content. This study is innovative in the functional analysis of the MADS family transcription factor in the metabolism of medium-chain fatty acids (MCFA) of oil palm, provides a certain research basis for improving the metabolic pathway of chain fatty acids in oil palm, and improves the synthesis of MCFA in plants. Our results will provide a reference direction for further research on improving the oil quality through biotechnology of oil palm.
摘要：
结论：EgMADS3，一个关键的转录因子，通过与EgLPAAT启动子结合正向调节MCFA的积累，提高油棕中果皮的脂质含量。脂质作为细胞膜的结构成分，作为细胞外部环境的渗透屏障。植物储存脂类中的中链脂肪酸是一种重要的可再生能源。植物脂质合成中MCFA生产的研究大多基于生化方法，转录调控在MCFA合成中的重要性及其在TAG中的掺入需要进一步研究。油棕是世界上产量最高的油料作物，在主要油料作物中产量最高。在这项研究中,表征了油棕中果皮中的MADS转录因子（EgMADS3）。通过VIGS病毒诱导的基因沉默,确定EgMADS3的潜在靶基因与中链脂肪酸（MCFA）的生物合成有关。原生质体瞬时转化和qRT-PCR分析表明EgMADS3正调控EgLPAAT的表达。酵母单杂交测定和EMSA的结果表明EgMADS3和EgLPAAT启动子之间的相互作用。通过遗传转化和脂肪酸分析,结论EgMADS3直接调控潜在靶基因EgLPAAT的中链脂肪酸合成途径,从而促进MCFA的积累并提高总脂质含量。本研究在油棕中链脂肪酸（MCFA）代谢中MADS家族转录因子的功能分析中具有创新性，为改善油棕中链状脂肪酸的代谢途径提供了一定的研究依据,并提高了植物中MCFA的合成。本研究结果将为进一步研究利用生物技术提高油棕油品质提供参考方向。
