Abstract：
Zeta potential is commonly referred as surface charge density and is a key factor in modulating the structural and functional properties of nucleic acids. Although the negative charge density of B-DNA is well understood, there is no prior description of the zeta potential measurement of Z-DNA. In this study, for the first time we discover the zeta potential difference between B-DNA and lanthanum chloride-induced Z-DNA. A series of linear repeat i.e. (CG)n and (GC)n DNA as well as branched DNA (bDNA) structures was used for the B-to-Z DNA transition. Herein, the positive zeta potential of Z-DNA has been demonstrated as a powerful tool to discriminate between B-form and Z-form of DNA. The generality of the approach has been validated both in linear and bDNA nanostructures. Thus, we suggest zeta potential can be used as an ideal signature for the left-handed Z-DNA.
摘要：
ζ电位通常被称为表面电荷密度,并且是调节核酸的结构和功能特性的关键因素。虽然B-DNA的负电荷密度是众所周知的,先前没有Z-DNA的ζ电位测量的描述。在这项研究中,我们证明了B-DNA和氯化镧诱导的Z-DNA的ζ电位之间的差异。将一系列线性重复即(CG)n/(GC)nDNA以及分支DNA(b-DNA)结构用于B-ZDNA转换。在这里，Z-DNA的正zeta电位已被证明是区分构象B-Z转变的有力工具。该方法的通用性已在线性和自组装DNA纳米结构中得到验证。因此，我们建议zeta电位可以用作左撇子Z-DNA的理想特征。
