PDF(Pubmed)
Abstract：
Xylene is the commonest clearing agent even though it is hazardous and costly. This study evaluated the clearing properties of coconut oil as an alternative cost-effective clearing agent for histological processes. Ten (10) prostate samples fixed in formalin were taken and each one was cut into 4 before randomly separating them into four groups (A, B, C and D). Tissues were subjected to ascending grades of alcohol for dehydration. Group A was cleared in xylene and Groups B, C, and D were cleared at varying times of 1hr 30mins, 3hrs, and 4hrs in coconut oil respectively before embedding, sectioning, and staining were carried out. Gross and histological features were compared. Results indicated a significant shrinkage in coconut oil-treated specimen compared with the xylene-treated specimen and only the tissues cleared in coconut oil for 4hrs were as rigid as the tissues cleared in xylene (p > 0.05). No significant difference was found in either of the sections when checked for cellular details and staining quality (p > 0.999). Coconut oil is an efficient substitute for xylene in prostate tissues with a minimum clearing time of 4hrs, as it is environmentally friendly and less expensive, but causes significant shrinkage to prostate tissue.
摘要：
二甲苯是最常见的清除剂，即使它是危险和昂贵的。这项研究评估了椰子油作为组织学过程的替代成本有效的清除剂的清除特性。取固定在福尔马林中的十（10）个前列腺样本，将每个样本切成4个，然后将它们随机分为四组（A，B,C和D）。对组织进行递增等级的酒精脱水。在二甲苯和B组中清除A组,C,和D在1小时30分钟的不同时间清除，3小时,在包埋前分别在椰子油中4小时，切片,并进行染色。比较了大体和组织学特征。结果表明，与二甲苯处理的样品相比，椰子油处理的样品明显收缩，只有在椰子油中清除4小时的组织与在二甲苯中清除的组织一样坚硬（p>0.05）。当检查细胞细节和染色质量时,在任一切片中均未发现显著差异(p>0.999)。椰子油是前列腺组织中二甲苯的有效替代品，最少清除时间为4小时，因为它环保且便宜，但导致前列腺组织明显收缩。
