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Abstract：
OBJECTIVE: Cystic echinococcosis (CE) is caused by the larval form of Echinococcus granulosus. Clinical, radiologic, pathologic, and serologic findings should be evaluated together for the diagnosis of CE. The sensitivity and specificity oalf serologic tests may vary depending on the method used. In this study, we aimed to detect IgG antibodies specific to E. granulosus using indirect hemagglutination assay (IHA), enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibodies (IFA) and western blot (WB) tests.
METHODS: In our study, the serum samples of 74 patients sent to our laboratory with suspicion of CE were studied using two different commercial IHA tests, ELISA, IFA and WB test. The test results were evaluated along with radiological findings and histopathological examinations, the latter being the gold standard.
RESULTS: Of all the patients, 51 (69%) were female and 23 (31%) were male. There was a statistically significant difference between males and females (χ2 = 9.7, p = 0.002). Out of 74 patients, positivity rates for Siemens IHA, Fumouze IHA, ELISA, IFA and WB test were positive as 33 (44.6%), 35 (47.3%), 43 (58.1%), 42 (56.7%) and 38 (51.3%), respectively. The sensitivity and specificity of the tests were as follows: 66.67 and 2.31% for Siemens IHA; 70.83% and 96.15% for Fumouze IHA; 85.42%, and 88.46% for ELISA; 83.33% and 88.46% for IFA; 72.92% and 88.46% for WB test.
CONCLUSIONS: There were statistically significant differences in between all five methods (p < 0,001). While the tests with the highest specificity was Fumouze IHA, the test with the highest sensitivity was the ELISA test. It was concluded that IHA and ELISA tests were more practical in practice because of their greater applicability.
METHODS: In our study, the serum samples of 74 patients sent to our laboratory with suspicion of CE were studied using two different commercial IHA tests, ELISA, IFA and WB test. The test results were evaluated along with radiological findings and histopathological examinations, the latter being the gold standard.
RESULTS: Of all the patients, 51 (69%) were female and 23 (31%) were male. There was a statistically significant difference between males and females (χ2 = 9.7, p = 0.002). Out of 74 patients, positivity rates for Siemens IHA, Fumouze IHA, ELISA, IFA and WB test were positive as 33 (44.6%), 35 (47.3%), 43 (58.1%), 42 (56.7%) and 38 (51.3%), respectively. The sensitivity and specificity of the tests were as follows: 66.67 and 2.31% for Siemens IHA; 70.83% and 96.15% for Fumouze IHA; 85.42%, and 88.46% for ELISA; 83.33% and 88.46% for IFA; 72.92% and 88.46% for WB test.
CONCLUSIONS: There were statistically significant differences in between all five methods (p < 0,001). While the tests with the highest specificity was Fumouze IHA, the test with the highest sensitivity was the ELISA test. It was concluded that IHA and ELISA tests were more practical in practice because of their greater applicability.
摘要：
目的：囊性包虫病（CE）是由细粒棘球蚴的幼虫形式引起的。临床,放射学,病理性,和血清学结果应一起评估以诊断CE。血清学检查的敏感性和特异性可能因所用方法而异。在这项研究中,我们的目的是使用间接血凝试验（IHA）来检测特异性针对细粒大肠杆菌的IgG抗体，酶联免疫吸附测定(ELISA),间接荧光抗体（IFA）和蛋白质印迹（WB）测试。
方法：在我们的研究中，使用两种不同的商业IHA测试研究了送往我们实验室怀疑CE的74名患者的血清样本，ELISA,IFA和WB测试。测试结果与放射学发现和组织病理学检查一起进行评估，后者是黄金标准。
结果：在所有患者中，51（69％）为女性，23（31％）为男性。男性和女性之间存在统计学差异（χ2=9.7，p=0.002）。在74名患者中，西门子IHA的阳性率，FumouzeIHA,ELISA,IFA和WB检测阳性为33(44.6%)，35（47.3%），43（58.1%），42（56.7%）和38（51.3%），分别。检测的敏感性和特异性如下:西门子IHA为66.67％和2.31％；FumouzeIHA为70.83％和96.15％；85.42％，ELISA检测为88.46%；IFA检测为83.33%和88.46%；WB检测为72.92%和88.46%。
结论：所有五种方法之间存在统计学上的显着差异（p<0,001）。虽然特异性最高的测试是FumouzeIHA，灵敏度最高的测试是ELISA测试。结论IHA和ELISA试验因其适用性较大而在实践中更实用。
方法：在我们的研究中，使用两种不同的商业IHA测试研究了送往我们实验室怀疑CE的74名患者的血清样本，ELISA,IFA和WB测试。测试结果与放射学发现和组织病理学检查一起进行评估，后者是黄金标准。
结果：在所有患者中，51（69％）为女性，23（31％）为男性。男性和女性之间存在统计学差异（χ2=9.7，p=0.002）。在74名患者中，西门子IHA的阳性率，FumouzeIHA,ELISA,IFA和WB检测阳性为33(44.6%)，35（47.3%），43（58.1%），42（56.7%）和38（51.3%），分别。检测的敏感性和特异性如下:西门子IHA为66.67％和2.31％；FumouzeIHA为70.83％和96.15％；85.42％，ELISA检测为88.46%；IFA检测为83.33%和88.46%；WB检测为72.92%和88.46%。
结论：所有五种方法之间存在统计学上的显着差异（p<0,001）。虽然特异性最高的测试是FumouzeIHA，灵敏度最高的测试是ELISA测试。结论IHA和ELISA试验因其适用性较大而在实践中更实用。
