Abstract：
Little is known about the immune response of lizards to Leishmania parasties. In this study, we conducted the first liver transcriptome analysis of two lizards (Phrynocephalus przewalskii and Eremias multiocellata) challenged with L. donovani, endemic to the steppe desert region of northwestern China. Our results revealed that multiple biological processes and immune-related signaling pathways are closely associated with the immune response to experimental L. donovani infection in the two lizards, and that both lizards show similar changes to mammals in terms of immunity to Leishmania. However, the interspecific divergence of the two lizards leads to different transcriptomic changes. In particular, in contrast to P. przewalskii, the challenged E. mutltiocellata was characterized by the induction of down-regulation of most DEGs. These findings will contribute to the scarce resources on lizard immunity and provide a reference for further research on immune mechanisms in reptiles.
摘要：
关于蜥蜴对利什曼原虫寄生虫的免疫反应知之甚少。在这项研究中,我们对受到多诺瓦尼乳杆菌攻击的两只蜥蜴（Phrynocalusprzewalskii和Eremiasmultiocellata）进行了首次肝脏转录组分析，中国西北草原荒漠地区特有。我们的研究结果表明，多种生物学过程和免疫相关的信号通路与两种蜥蜴对实验性多诺瓦尼乳杆菌感染的免疫反应密切相关。两种蜥蜴在对利什曼原虫的免疫力方面表现出与哺乳动物相似的变化。然而,两种蜥蜴的种间差异导致不同的转录组变化。特别是,与P.przewalskii相比，受到挑战的多叶大肠杆菌的特征是诱导大多数DEGs的下调。这些发现将有助于对蜥蜴免疫的稀缺资源,并为进一步研究爬行动物的免疫机制提供参考。
