PDF(Pubmed)
Abstract：
Sepsis is a life-threatening condition characterized by a systemic inflammatory response to infection. Despite extensive research on its pathophysiology, effective therapeutic approaches remain a challenge. This study investigated the potential of resveratrol (RV) and silver nanoparticle-enhanced resveratrol (AgNP-RV) as treatments for sepsis-induced lung injury using a rat model of polymicrobial sepsis induced by cecal ligation and puncture (CLP). The study focused on evaluating changes in oxidative status (TAS, TOS, and OSI) and the expression of inflammatory and apoptotic markers (IL-1β, TNF-α, P2X7R, TLR4, Caspase-3, and Bcl-2) in lung tissue. Both RV and AgNP-RV demonstrated potential in mitigating oxidative stress, inflammation, and apoptosis, with AgNP-RV exhibiting greater efficacy than RV alone (p < 0.05). These findings were corroborated by histopathological analyses, which revealed reduced tissue damage in the RV- and AgNP-RV-treated groups. Our study highlights the therapeutic potential of RV and, particularly, AgNP-RV in combating sepsis-induced oxidative stress, inflammation, and apoptosis. It also underscores the promise of nanoparticle technology in enhancing therapeutic outcomes. However, further investigations are warranted to fully understand the mechanisms of action, especially concerning the role of the P2X7 receptor in the observed effects. Nonetheless, our research suggests that RV and AgNP-RV hold promise as novel strategies for sepsis management.
摘要：
脓毒症是一种威胁生命的疾病，其特征是对感染的全身性炎症反应。尽管对其病理生理学进行了广泛的研究，有效的治疗方法仍然是一个挑战。这项研究调查了白藜芦醇（RV）和银纳米颗粒增强的白藜芦醇（AgNP-RV）作为使用盲肠结扎和穿孔（CLP）诱导的多微生物脓毒症大鼠模型的脓毒症诱导的肺损伤的治疗潜力。这项研究的重点是评估氧化状态的变化(TAS，TOS,和OSI）以及炎症和凋亡标志物（IL-1β，TNF-α,P2X7R,肺组织中的TLR4、Caspase-3和Bcl-2)。RV和AgNP-RV均显示出减轻氧化应激的潜力，炎症,和细胞凋亡，AgNP-RV表现出比单独RV更大的功效(p<0.05)。组织病理学分析证实了这些发现，这表明RV和AgNP-RV治疗组的组织损伤减少。我们的研究强调了RV的治疗潜力，特别是,AgNP-RV在对抗脓毒症诱导的氧化应激中，炎症,和凋亡。它还强调了纳米粒子技术在提高治疗效果方面的前景。然而,需要进一步调查，以充分了解行动机制，特别是关于P2X7受体在观察到的作用中的作用。尽管如此，我们的研究表明,RV和AgNP-RV有望成为脓毒症管理的新策略.
