PDF(Pubmed)
Abstract：
Given the growing importance of lab-on-a-chip in a number of fields, such as medical diagnosis or environmental analysis, the fact that the current fabrication process relies mainly on oil-based polymers raises an ecological concern. As an eco-responsible alternative, we presented, in this article, a manufacturing process for microfluidic devices from chitosan, a bio-sourced, biodegradable, and biocompatible polysaccharide. From chitosan powder, we produced thick and rigid films. To prevent their dissolution and reduce their swelling when in contact with aqueous solutions, we investigated a film neutralization step and characterized the mechanical and physical properties of the resulting films. On these neutralized chitosan films, we compared two micropatterning methods, i.e., hot embossing and mechanical micro-drilling, based on the resolution of microchannels from 100 µm to 1000 µm wide. Then, chitosan films with micro-drilled channels were bonded using a biocompatible dry photoresist on a glass slide or another neutralized chitosan film. Thanks to this protocol, the first functional chitosan microfluidic devices were prepared. While some steps of the fabrication process remain to be improved, these preliminary results pave the way toward a sustainable fabrication of lab-on-a-chip.
摘要：
鉴于实验室在许多领域的重要性日益增加，如医学诊断或环境分析，事实上，目前的制造过程主要依赖于油基聚合物引起了生态问题。作为一种对生态负责的选择，我们提出，在这篇文章中,从壳聚糖制造微流体装置的过程，生物来源的，可生物降解，和生物相容性多糖。从壳聚糖粉末，我们生产了厚而硬的薄膜。为了防止它们溶解并减少与水溶液接触时的膨胀，我们研究了薄膜中和步骤，并表征了所得薄膜的机械和物理性能。在这些中和的壳聚糖膜上，我们比较了两种微图案化方法，即，热压花和机械微钻孔，基于从100µm到1000µm宽的微通道的分辨率。然后，将具有微钻孔通道的壳聚糖膜使用生物相容的干光致抗蚀剂粘合在载玻片或另一种中和的壳聚糖膜上。多亏了这个协议，首先制备了功能性壳聚糖微流控器件。虽然制造工艺的一些步骤仍有待改进，这些初步结果为芯片上实验室的可持续制造铺平了道路。
