PDF(Pubmed)
Abstract：
Diabetes mellitus (DM) is the second leading cause of mortality globally. The increased concern for DM is due to the underlying complications accompanying hyperglycaemia, associated with oxidative stress and consequent inflammation. The investigation of safe and effective treatments for DM is necessary. In the present study, the cytotoxicity, phytochemical analysis, antioxidant capacity, anti-inflammatory, and antidiabetic effects in an aqueous extract of Garcinia livingstonei leaves were assessed. All tested extract concentrations showed no toxicity against C3A hepatocytes. Several phenolic compounds were identified using ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS). The total polyphenol content was 100.9741 mg GAE/g, 16.7712 mg CE/g flavanols, and 2.3548 mg QE/g flavonols. The antioxidant capacity values were 253.4268 mg AAE/g, 192.232 mg TE/g, and 167.8724 mg TE/g for ferric reducing antioxidant power (FRAP), Trolox equivalent antioxidant capacity (TEAC), and 2,2-diphenyl-1-pycrylhydrazyl (DPPH), respectively. The plant extract significantly (p < 0.05) demonstrated anti-inflammatory and hypoglycaemic effects in a dose-dependent manner, with the α-glucosidase inhibition of the extract being higher (p < 0.05) than in the standard conventional drug (acarbose). The findings of this study revealed the potential of the constituents of G. livingstonei aqueous leaf extract in DM treatment. Further studies on the preparation and mechanisms of action of the plant in DM treatment are recommended.
摘要：
糖尿病（DM）是全球第二大死亡原因。对DM的关注增加是由于伴随高血糖的潜在并发症，与氧化应激和随之而来的炎症有关。有必要研究安全有效的DM治疗方法。在本研究中,细胞毒性，植物化学分析,抗氧化能力，抗炎,并评估了藤黄藤黄叶的水提取物中的抗糖尿病作用。所有测试的提取物浓度显示对C3A肝细胞没有毒性。使用超高效液相色谱质谱（UHPLC-MS）鉴定了几种酚类化合物。总多酚含量为100.9741mgGAE/g，16.7712毫克CE/克黄烷醇，和2.3548mgQE/g黄酮醇。抗氧化能力值为253.4268mgAAE/g，192.232mgTE/g，和167.8724毫克TE/克的铁还原抗氧化能力（FRAP），Trolox等效抗氧化能力（TEAC），和2,2-二苯基-1-吡啶基肼基(DPPH)，分别。植物提取物显着（p<0.05）以剂量依赖性方式显示抗炎和降血糖作用，提取物的α-葡萄糖苷酶抑制高于(p<0.05)标准常规药物(阿卡波糖)。这项研究的发现揭示了G.livingstonei水性叶提取物成分在DM治疗中的潜力。建议对DM处理中植物的制备和作用机理进行进一步研究。
