Abstract：
Photodynamic inactivation (PDI) technology is a promising alternative to antibiotics. This technology is defined as the inhibition of bacterial growth with photosensitizers while irradiated with low-level laser light in the wavelength of 532 ± 2.08 nm. A challenging area in this field is selecting photosensitizers with antibacterial potential. In this paper, to enhance the antibacterial efficiency, the photosensitizers (the selected plant extracts) with a high absorption peak at the selected laser frequency, 532 nm, were prepared. Low-concentration ethanolic plant extracts of Hibiscus sabdariffa and Opuntia ficus-indica were found to exhibit significant antibacterial activity against, Acinetobacter baumannii ATCC 19606 and, Staphylococcus aureus ATCC 33591 as two important human pathogenic bacteria. The effectiveness of these natural photosensitizers was measured by determining their Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values and by performing a time-killing assay in the absence and the presence of laser irradiation. Our results showed that the combination of low-level laser irradiation and the selected photosensitizers had excellent potential for treating in vitro bacterial infections. Therefore, PDI technology has great potential as a viable alternative to traditional antibiotics for combating bacterial infections. This study presents a promising avenue for further exploration of PDI and the use of laser technology in medical science.
摘要：
光动力灭活（PDI）技术是一种有前途的抗生素替代品。该技术定义为在用波长为532±2.08nm的低水平激光照射时用光敏剂抑制细菌生长。该领域的一个挑战性领域是选择具有抗菌潜力的光敏剂。在本文中,为了提高抗菌效率，光敏剂（选定的植物提取物）在选定的激光频率下具有高吸收峰，532nm,准备好了。发现木芙蓉和仙人掌的低浓度乙醇植物提取物对，鲍曼不动杆菌ATCC19606和，金黄色葡萄球菌ATCC33591为两种重要的人类致病菌。这些天然光敏剂的有效性通过测定它们的最小抑制浓度(MIC)和最小杀菌浓度(MBC)值并通过在不存在和存在激光照射的情况下进行时间杀灭测定来测量。我们的结果表明，低水平激光照射和选定的光敏剂的组合具有治疗体外细菌感染的极好潜力。因此,PDI技术作为对抗细菌感染的传统抗生素的可行替代品具有巨大潜力。这项研究为进一步探索PDI和激光技术在医学科学中的应用提供了有希望的途径。
