■湖羊是一种以其高繁殖率而闻名的品种。一年四季都在发情期,其繁殖种群正在逐步扩大。然而,目前冷冻保存精液的技术效果有限,这阻碍了该物种的持续发展。目的探讨不同穿透性冷冻保护剂(CPAs)和蛋黄(EY)浓度对湖羊精液冷冻保存效果的影响,以确定最有效的组合。
■在这项研究中,甘油(GLY)的影响,乙二醇(EG),二甲基乙酰胺(DMA),二甲基亚砜(DMSO),不同比例的GLY和EG,通过检测精子总运动性(TM),研究EY对解冻后精子质量的影响,渐进运动(PM),直线速度(VSL),曲线速度(VCL),平均路径速度(VAP),头部侧向位移(ALH)的振幅,摆动运动系数(WOB),平均运动度(MAD),功能完整性(质膜完整性,顶体完整性)和活性氧(ROS)水平。
■当GLY和EG加在一起时,与其他浓度组相比,6%GLY显著(p<0.05)增加TM,PM,质膜完整性,和解冻精子的顶体完整性。此外,显著降低精子ROS水平(p<0.05)。在这项研究中,TM,6%EG的PM和膜完整性显著高于对照(p<0.05),1%GLY+5%EG和6%GLY+6%EG组。与其他浓度组相比,20%EY显著(p<0.05)提高了TM,PM,和解冻精子的质膜完整性。然而,顶体的完整性随着EY浓度的增加而增加。
■总而言之,与其他组相比,用含有6%GLY和20%EY的稀释剂稀释的解冻后的Huram精液表现出更高的质量。
UNASSIGNED: The Hu sheep is a renowned breed known for its high reproductive rate. It is in estrus all year round, and its breeding population is gradually expanding. However, the current techniques for cryopreserving semen have limited effectiveness, which hinders the continuous development of this species. The purpose of this study is to explore the effects of different penetrating cryoprotectants (CPAs) and egg yolk (EY) concentrations on the cryopreservation of Hu ram semen to determine the most effective combination.
UNASSIGNED: In this study, the effects of glycerol (GLY), ethylene glycol (EG), dimethylacetamide (DMA), dimethyl sulfoxide (DMSO), different proportions of GLY and EG, EY on sperm quality after thawing were investigated by detecting sperm total motility (TM), progressive motility (PM), straight-line velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), amplitude of lateral head displacement (ALH), wobble movement coefficient (WOB), average motion degree (MAD), functional integrity (plasma membrane integrity, acrosome integrity) and reactive oxygen species (ROS) level.
UNASSIGNED: When GLY and EG were added together, compared to other concentration groups, 6% GLY significantly (p < 0.05) increased TM, PM, plasma membrane integrity, and acrosome integrity of thawed sperm. Additionally, it significantly (p < 0.05) decreased the ROS level of sperm. In this study, the TM, PM and membrane integrity of the 6% EG were significantly (p < 0.05) higher than those of the control, 1% GLY+5% EG and 6% GLY+6% EG groups. Compared to other concentration groups, 20% EY significantly (p < 0.05) improved the TM, PM, and plasma membrane integrity of thawed sperm. However, the integrity of the acrosome increased with the higher concentration of EY.
UNASSIGNED: In conclusion, the post-thawed Hu ram semen diluted with a diluent containing 6% GLY and 20% EY exhibited higher quality compared to the other groups.