PDF(Pubmed)
Abstract：
During the current investigation, eight essential oils (EOs) were tested for their antimicrobial activity against six species, belonging to the genus of staphylococcus, multi-resistant to antibiotics (S. epidermidis, S. cohni, S. wareneri, S. scuiri, S. chromogenes, S. pasteuri), three methicillin-resistant Staphylococcus aureus strains (MRSA) and two strains of Escherichia coli, producing extended-spectrum β-lactamase (ESBL) responsible for bovine mastitis. Our results indicated that the antimicrobial activities of eight EOs varied significantly among the types of EOs and bacterial species. Thymus capitatus and Trachyspermum ammi EOs display important antibacterial activity against all tested strains, with the inhibition zone diameters situated between 20 and 45 mm, while EOs of Artemisia absinthium, Eucalyptus globulus, Eucalyptus camaldulensis, Myrtus communis and Mentha pulegium exerted an intermediate activity. For Cymbopogon citratus, this effect depends on bacteria species. In fact, an important effect was observed against S. warneri, S. epidermidis, S. cohenii, S. pasteuri and MRSA (EC 39+) strains. In addition, the important lytic effect was observed against MRSA strains, showing that Gram-positive bacteria were more sensitive to T. capitatus EO than Gram-negative ones. Concerning the characterization of the mode action of T. capitatus, experiments of kill-time, bacteriolytic, loss of salt tolerance and loss of cytoplasmic material showed that the used EO was able to destroy cell walls and membranes followed by the loss of vital intracellular materials. In addition, it inhibits the normal synthesis of DNA, causing the bacterial death of E. coli and MRSA strains. This study shows the potential of using of EOs, particularly T. capitaus, to inhibit the growth of Gram-positive and Gram-negative bacteria multi-resistant to antibiotics causing bovine mastitis.
摘要：
在目前的调查中,测试了八种精油（EO）对六种物种的抗菌活性，属于葡萄球菌属，对抗生素具有多重耐药性(S.表皮,S.Cohni,S.Wareneri，S、Scuiri，S、染色体基因，S.巴斯德里)，三株耐甲氧西林金黄色葡萄球菌(MRSA)和两株大肠杆菌,产生引起牛乳腺炎的超广谱β-内酰胺酶（ESBL）。我们的结果表明，八种EO的抗菌活性在EO的类型和细菌种类之间存在显着差异。Thymuscapatus和TrachyspermumammiEOs对所有测试菌株均具有重要的抗菌活性，抑制区直径在20到45毫米之间，而苦艾蒿的EO，球桉树，桉树，Myrtuscommunis和Menthapulegium发挥了中间活动。对于Cymboponcitratus，这种效果取决于细菌种类。事实上,观察到对S.Warneri的重要影响，表皮葡萄球菌，S、cohenii，巴氏菌和MRSA(EC39+)菌株。此外,对MRSA菌株观察到重要的裂解作用，表明革兰氏阳性菌对T.capatusEO比革兰氏阴性菌更敏感。关于T.capatus模式作用的表征，消磨时间的实验，溶菌,耐盐性的丧失和细胞质物质的丧失表明，使用的EO能够破坏细胞壁和细胞膜，然后丢失重要的细胞内物质。此外,抑制DNA的正常合成,导致大肠杆菌和MRSA菌株的细菌死亡。这项研究显示了使用EO的潜力，特别是T.capetaus,以抑制革兰氏阳性和革兰氏阴性细菌的生长，这些细菌对引起牛乳腺炎的抗生素具有多重耐药性。
