Abstract：
Cryptosporidium spp. and G. duodenalis often infect humans, cats, and other mammals, causing diarrhea and being responsible for numerous outbreaks of waterborne and foodborne infections worldwide. The rapid increase in the number of pet cats poses a substantial public health risk. However, there were few reports about the infection of Cryptosporidium spp. and G. duodenalis infections in pet cats in Henan Province, central China. Thus, to understand the prevalence and genetic distribution of Cryptosporidium spp. and G. duodenalis in pet cats, and to evaluate the zoonotic potential, possible transmission routes and public health implications of isolates, fecal samples (n = 898) were randomly collected from pet cats in 11 cities in Henan Province, central China. Nested PCR based on the SSU rRNA gene and bg gene was used to the prevalence of Cryptosporidium spp. and G. duodenalis, respectively. The prevalence was 0.8 % (7/898) and 2.0 % (18/898) for Cryptosporidium spp. and G. duodenalis respectively. Additionally, the Cryptosporidium spp. positive isolates were identified as C. parvum subtype IIdA19G1 by gp60 gene. In the present study, the IIdA19G1 subtype was discovered in pet cats for the first time in China, enriching the information on the host type and geographical distribution of Cryptosporidium spp. in China. For G. duodenalis, a total of 18 G. duodenalis positive samples were identified, belonging to four assemblages: a zoonotic assemblage A1 (4/898), three host-specific assemblages C (8/898), D (5/898), and F (1/898). Interestingly, we found that pet cats infected with Cryptosporidium spp. and G. duodenalis are more likely to experience emaciation symptoms compared to the negative group. More importantly, the prevalence of Cryptosporidium spp. and G. duodenalis detected in the present study were low, but the subtype IIdA19G1 of Cryptosporidium spp. and the assemblages A1, C, D, and F of G. duodenalis have the potential for zoonotic transmission. Thus, we should focus on preventing and controlling the risk of cross-species transmission that may occur in pet cats in Henan Province.
摘要：
隐孢子虫。十二指肠G.经常感染人类，猫,和其他哺乳动物，引起腹泻，并在全球范围内多次爆发水传播和食源性感染。宠物猫数量的迅速增加带来了巨大的公共卫生风险。然而,关于隐孢子虫感染的报道很少。河南省宠物猫的十二指肠球部感染，中国中部。因此，目的了解隐孢子虫的流行情况和基因分布。和宠物猫中的G.daidenalis，并评估人畜共患的潜力，分离株可能的传播途径和公共卫生影响，从河南省11个城市的宠物猫中随机采集粪便样本（n=898），中国中部。基于SSUrRNA基因和bg基因的巢式PCR用于隐孢子虫的流行。和十二指肠G，分别。隐孢子虫的患病率为0.8％（7/898）和2.0％（18/898）。和十二指肠G.此外,隐孢子虫属。阳性分离株通过gp60基因鉴定为C.parvum亚型IIdA19G1。在本研究中,在中国首次在宠物猫中发现了IIdA19G1亚型，丰富了隐孢子虫的寄主类型和地理分布信息。在中国。对于十二指肠氏杆菌，共鉴定出18份十二指肠G.阳性样本，属于四个组合：人畜共患组合A1（4/898），三个宿主特异性组合C(8/898)，D(5/898),和F（1/898）。有趣的是,我们发现宠物猫感染了隐孢子虫.与阴性组相比，十二指肠氏杆菌更有可能出现消瘦症状。更重要的是,隐孢子虫的患病率。在本研究中检测到的十二指肠G。而是隐孢子虫的IIdA19G1亚型。和组合A1，C，D,和十二指肠G的F具有人畜共患传播的潜力。因此，重点防控河南省宠物猫可能发生的跨物种传播风险。
