Abstract：
Integration of hepatitis B virus (HBV) DNA into the human genome is recognized as an oncogenic factor and a barrier to hepatitis B cure. In the study, biopsy liver tissues were collected from adolescents and young adults with acute HBV infection younger than or equal to 35 years of age and from HBV-infected infant patients younger than or equal to 6 months of age. A high-throughput sequencing method was used to detect HBV DNA integration. Totally, 12 adolescents, young adults, and 6 infants were included. Among the 12 patients with acute HBV infection, immunohistochemical staining of intrahepatic hepatitis B surface antigen for all displayed negative results, and no HBV DNA integrants in the hepatocyte DNA were confirmed. All infant patients had elevated levels of alanine aminotransferase and high levels of serum HBV DNA. Numerous gene sites of hepatocyte DNA were integrated by HBV DNA for each infant patient, ranging from 120 to 430 integration sites. The fragile histidine triad gene was the high-frequency integrated site in the intragenic region for infant patients. In conclusion, hepatocyte DNA is integrated by HBV DNA in babies with active hepatitis B but seems seldom affected among adolescents and young adults with acute HBV infection. Infantile hepatitis B should be taken seriously considering abundant HBV DNA integration events.
摘要：
将乙型肝炎病毒（HBV）DNA整合到人类基因组中被认为是致癌因子和乙型肝炎治愈的障碍。在研究中,活检肝组织来自年龄小于或等于35岁的急性HBV感染青少年和年轻成人,以及年龄小于或等于6个月的HBV感染婴儿患者.采用高通量测序方法检测HBVDNA整合。完全正确,12名青少年,年轻人,包括6名婴儿。在12例急性HBV感染患者中，所有显示阴性结果的肝内乙型肝炎表面抗原的免疫组织化学染色，肝细胞DNA中未证实HBVDNA整合体。所有婴儿患者的丙氨酸转氨酶水平升高，血清HBVDNA水平升高。肝细胞DNA的许多基因位点被HBVDNA整合为每个婴儿患者，范围从120到430集成网站。脆性组氨酸三联体基因是婴儿患者基因内区域的高频整合位点。总之,肝细胞DNA被HBVDNA整合在婴儿活动性乙型肝炎，但似乎很少影响青少年和年轻成人急性HBV感染。考虑到丰富的HBVDNA整合事件，应认真对待婴儿乙型肝炎。
