PDF(Pubmed)
Abstract：
UNASSIGNED: Carbapenem-resistant Klebsiella pneumoniae (CRKP) clinical isolations have rapidly increased in pediatric patients. To investigate a possible health care-associated infections of CRKP in a tertiary pediatric hospital, the circulating clones and carbapenem-resistant pattern between CRKP and carbapenem-resistant Acinetobacter baumannii (CRAB) isolates were compared to classify their epidemiological characteristics. The results will help to identify the epidemic pattern of the CRKP transmission in the hospital.
UNASSIGNED: Ninety-six CRKP and forty-eight CRAB isolates were collected in Kunming Children\'s Hospital from 2019 through 2022. These isolates were genotyped using repetitive extragenic palindromic-PCR (REP-PCR). Carbapenemase phenotypic and genetic characterization were investigated using a disk diffusion test and singleplex PCR, respectively. In addition, these characteristics of the two pathogens were compared.
UNASSIGNED: The rates of CRKP and CRAB ranged from 15.8% to 37.0% at the hospital. Forty-nine and sixteen REP genotypes were identified among the 96 and 48 CRKP and CRAB isolates tested, respectively. The CRKP isolates showed more genetic diversity than the CRAB isolates. Of the 96 CRKP isolates, 69 (72%) produced Class B carbapenemases. However, all 48 CRAB isolates produced Class D carbapenemase or extended-spectrum β-lactamases (ESBL) combined with the downregulation of membrane pore proteins. Furthermore, the carbapenemase genes bla KPC, bla IMP, and bla NDM were detected in CRKP isolates. However, CRAB isolates were all positive for the bla VIM, bla OXA-23, and bla OXA-51 genes.
UNASSIGNED: These CRKP isolates exhibited different biological and genetic characteristics with dynamic changes, suggesting widespread communities. Continuous epidemiological surveillance and multicenter research should be carried out to strengthen the prevention and control of infections.
UNASSIGNED: Ninety-six CRKP and forty-eight CRAB isolates were collected in Kunming Children\'s Hospital from 2019 through 2022. These isolates were genotyped using repetitive extragenic palindromic-PCR (REP-PCR). Carbapenemase phenotypic and genetic characterization were investigated using a disk diffusion test and singleplex PCR, respectively. In addition, these characteristics of the two pathogens were compared.
UNASSIGNED: The rates of CRKP and CRAB ranged from 15.8% to 37.0% at the hospital. Forty-nine and sixteen REP genotypes were identified among the 96 and 48 CRKP and CRAB isolates tested, respectively. The CRKP isolates showed more genetic diversity than the CRAB isolates. Of the 96 CRKP isolates, 69 (72%) produced Class B carbapenemases. However, all 48 CRAB isolates produced Class D carbapenemase or extended-spectrum β-lactamases (ESBL) combined with the downregulation of membrane pore proteins. Furthermore, the carbapenemase genes bla KPC, bla IMP, and bla NDM were detected in CRKP isolates. However, CRAB isolates were all positive for the bla VIM, bla OXA-23, and bla OXA-51 genes.
UNASSIGNED: These CRKP isolates exhibited different biological and genetic characteristics with dynamic changes, suggesting widespread communities. Continuous epidemiological surveillance and multicenter research should be carried out to strengthen the prevention and control of infections.
摘要：
■耐碳青霉烯类肺炎克雷伯菌（CRKP）的临床分离在儿科患者中迅速增加。调查某三级儿科医院可能发生的CRKP卫生保健相关感染,比较了CRKP和耐碳青霉烯类鲍曼不动杆菌(CRAB)分离株的循环克隆和耐碳青霉烯类耐药模式,以对其流行病学特征进行分类.研究结果将有助于确定CRKP在医院传播的流行模式。
■从2019年至2022年，在昆明儿童医院收集了96株CRKP和48株CRAB分离株。使用重复基因外回文PCR（REP-PCR）对这些分离株进行基因分型。使用圆盘扩散测试和单重PCR研究了碳青霉烯酶的表型和遗传特征，分别。此外,对这两种病原菌的特征进行了比较。
■CRKP和CRAB在医院的发生率为15.8%至37.0%。在测试的96和48个CRKP和CRAB分离株中鉴定出49个和16个REP基因型，分别。CRKP分离株比CRAB分离株表现出更多的遗传多样性。在96个CRKP分离株中，69(72%)产生B类碳青霉烯酶。然而,所有48个CRAB分离株均产生D类碳青霉烯酶或超广谱β-内酰胺酶(ESBL),并下调膜孔蛋白.此外，碳青霉烯酶基因blaKPC，blaIMP,在CRKP分离株中检测到blaNDM。然而,CRAB分离株对BlaVIM均呈阳性，blaOXA-23和blaOXA-51基因。
■这些CRKP分离株表现出不同的生物学和遗传特性，并具有动态变化，建议广泛的社区。应开展持续的流行病学监测和多中心研究，加强感染的预防和控制。
■从2019年至2022年，在昆明儿童医院收集了96株CRKP和48株CRAB分离株。使用重复基因外回文PCR（REP-PCR）对这些分离株进行基因分型。使用圆盘扩散测试和单重PCR研究了碳青霉烯酶的表型和遗传特征，分别。此外,对这两种病原菌的特征进行了比较。
■CRKP和CRAB在医院的发生率为15.8%至37.0%。在测试的96和48个CRKP和CRAB分离株中鉴定出49个和16个REP基因型，分别。CRKP分离株比CRAB分离株表现出更多的遗传多样性。在96个CRKP分离株中，69(72%)产生B类碳青霉烯酶。然而,所有48个CRAB分离株均产生D类碳青霉烯酶或超广谱β-内酰胺酶(ESBL),并下调膜孔蛋白.此外，碳青霉烯酶基因blaKPC，blaIMP,在CRKP分离株中检测到blaNDM。然而,CRAB分离株对BlaVIM均呈阳性，blaOXA-23和blaOXA-51基因。
■这些CRKP分离株表现出不同的生物学和遗传特性，并具有动态变化，建议广泛的社区。应开展持续的流行病学监测和多中心研究，加强感染的预防和控制。
