Abstract：
In Dermanyssus gallinae, a hematophagous mite, the initiation of vitellogenesis induced by blood feeding is essential for its reproduction. However, the precise gene structures and physiological functions of Vg in D. gallinae and its upstream gene, Target of Rapamycin (TOR), have not been fully understood. This study revealed the presence of four homologous genes within D. gallinae, named Dg-Vg1, Dg-Vg1-like, Dg-Vg2, and Dg-Vg2-like, especially, Dg-Vg2-like was firstly identified in the mites. The expression levels of all these Vg genes were significantly higher in adult females than other stages. Following blood feeding, the expression levels of these genes increased significantly, followed by a subsequent decrease, aligning with egg production. Silencing Dg-Vgs by RNA interference (RNAi) led to decreased fecundity and egg hatching rates, as well as abnormal embryonic development, suggesting a vital role for Dg-Vgs in both egg formation and embryonic development. Furthermore, the knockdown of Dg-TOR significantly reduced the expression of Dg-Vgs and negatively impacted the reproductive capabilities of PRMs, indicating that TOR influences PRM reproduction by regulating the expression of Dg-Vgs. In summary, these findings demonstrated the crucial roles of Dg-Vgs and Dg-TOR in PRM reproduction, highlighting their potential as targets for pest control.
摘要：
在皮氏，吸血螨,血液喂养引起的卵黄发生的启动对其繁殖至关重要。然而,Gallinae及其上游基因中Vg的精确基因结构和生理功能，雷帕霉素靶蛋白(TOR),还没有被完全理解。这项研究揭示了D.gallinae中存在四个同源基因，命名为Dg-Vg1，类似Dg-Vg1，Dg-Vg2,Dg-Vg2,尤其是,首先在螨虫中鉴定出Dg-Vg2样。所有这些Vg基因的表达水平在成年雌性中明显高于其他阶段。采血后，这些基因的表达水平显著增加，随后下降，与鸡蛋生产保持一致。通过RNA干扰（RNAi）沉默Dg-Vgs导致繁殖力和卵孵化率降低，以及异常的胚胎发育，提示Dg-Vgs在卵形成和胚胎发育中的重要作用。此外，Dg-TOR的敲减会显著降低Dg-Vgs的表达，并对PRM的繁殖能力产生负面影响，表明TOR通过调节Dg-Vgs的表达影响PRM繁殖。总之,这些发现证明了Dg-Vgs和Dg-TOR在PRM繁殖中的关键作用，强调它们作为虫害防治目标的潜力。
