PDF(Pubmed)
Abstract：
UNASSIGNED: Candida albicans is one of the most common pathogenic yeasts, responsible for causing candidiasis. The use of conventional antifungal agents for the treatment of Candida is reported to be less effective and hence alternative therapies for the treatment are needed. Essential oils of medicinal plants may serve as a strong candidate for natural products in modern therapies.
UNASSIGNED: The aim of this study was to determine the synergistic potential of essential oils extracted from leaves of Aegle marmelos (L.) Correa and a potent antifungal agent, nystatin, against three clinical isolates of C. albicans using checkerboard assay.
UNASSIGNED: The antifungal activity of the essential oils of A. marmelos was screened against test cultures by disc diffusion technique. Antibiograms of the test organisms were developed. To determine the minimum fungicidal concentration of the essential oil and nystatin, the broth microdilution method was employed, and a checkerboard assay was used to investigate the synergistic potential of the essential oil and nystatin against the clinical isolates under study. The data were expressed as mean ± standard deviation.
UNASSIGNED: The Σ fractional inhibitory concentration values were calculated as 0.12, 0.37, and 0.28 for three different strains of C. albicans used, respectively, which was <0.5, therefore, the synergy was demonstrated between essential oils and nystatin against the test cultures.
UNASSIGNED: Combinatorial therapy of the essential oils extracted from the leaves of A. marmelos and nystatin may be considered a line of treatment for candidal infections.
UNASSIGNED: The aim of this study was to determine the synergistic potential of essential oils extracted from leaves of Aegle marmelos (L.) Correa and a potent antifungal agent, nystatin, against three clinical isolates of C. albicans using checkerboard assay.
UNASSIGNED: The antifungal activity of the essential oils of A. marmelos was screened against test cultures by disc diffusion technique. Antibiograms of the test organisms were developed. To determine the minimum fungicidal concentration of the essential oil and nystatin, the broth microdilution method was employed, and a checkerboard assay was used to investigate the synergistic potential of the essential oil and nystatin against the clinical isolates under study. The data were expressed as mean ± standard deviation.
UNASSIGNED: The Σ fractional inhibitory concentration values were calculated as 0.12, 0.37, and 0.28 for three different strains of C. albicans used, respectively, which was <0.5, therefore, the synergy was demonstrated between essential oils and nystatin against the test cultures.
UNASSIGNED: Combinatorial therapy of the essential oils extracted from the leaves of A. marmelos and nystatin may be considered a line of treatment for candidal infections.
摘要：
■白色念珠菌是最常见的致病性酵母菌之一，负责引起念珠菌病。据报道,使用常规抗真菌剂治疗念珠菌的效果较差,因此需要用于治疗的替代疗法。药用植物精油可以作为现代疗法中天然产物的强大候选物。
■这项研究的目的是确定从Aeglemarmelos的叶子中提取的精油的协同潜力（L.)Correa和一种有效的抗真菌剂,制霉菌素,使用棋盘法检测三种白色念珠菌的临床分离株。
■通过圆盘扩散技术针对测试培养物筛选A.marmelos精油的抗真菌活性。开发了测试生物体的抗生物图。为了确定精油和制霉菌素的最低杀菌浓度，采用肉汤微量稀释法，并使用棋盘分析法研究精油和制霉菌素对研究中的临床分离株的协同潜力。数据表示为平均值±标准偏差。
■对于使用的三种不同的白色念珠菌菌株，Σ分数抑制浓度值计算为0.12、0.37和0.28，分别,这是<0.5，因此，证明了精油和制霉菌素对测试培养物的协同作用。
■从A.marmelos和制霉菌素的叶子中提取的精油的组合疗法可以被认为是念珠菌感染的治疗路线。
■这项研究的目的是确定从Aeglemarmelos的叶子中提取的精油的协同潜力（L.)Correa和一种有效的抗真菌剂,制霉菌素,使用棋盘法检测三种白色念珠菌的临床分离株。
■通过圆盘扩散技术针对测试培养物筛选A.marmelos精油的抗真菌活性。开发了测试生物体的抗生物图。为了确定精油和制霉菌素的最低杀菌浓度，采用肉汤微量稀释法，并使用棋盘分析法研究精油和制霉菌素对研究中的临床分离株的协同潜力。数据表示为平均值±标准偏差。
■对于使用的三种不同的白色念珠菌菌株，Σ分数抑制浓度值计算为0.12、0.37和0.28，分别,这是<0.5，因此，证明了精油和制霉菌素对测试培养物的协同作用。
■从A.marmelos和制霉菌素的叶子中提取的精油的组合疗法可以被认为是念珠菌感染的治疗路线。
