PDF(Pubmed)
Abstract：
Pearl millet (Pennisetum glaucum (L.)) is a vital cereal crop renowned for its ability to thrive in challenging environmental conditions; however, the molecular mechanisms governing its salt stress tolerance remain poorly understood. To address this gap, next-generation RNA sequencing was conducted to compare gene expression patterns in pearl millet seedlings exposed to salt stress with those grown under normal conditions. Our RNA sequencing analysis focused on shoots from 13-day-old pearl millet plants subjected to either salinity stress (150 mmol of NaCl for 3 days) or thermal stress (50°C for 60 s). Of 36,041 genes examined, 17,271 genes with fold changes ranging from 2.2 to 19.6 were successfully identified. Specifically, 2388 genes were differentially upregulated in response to heat stress, whereas 4327 genes were downregulated. Under salt stress conditions, 2013 genes were upregulated and 4221 genes were downregulated. Transcriptomic analysis revealed four common abiotic KEGG pathways that play crucial roles in the response of pearl millet to salt and heat stress: phenylpropanoid biosynthesis, photosynthesis-antenna proteins, photosynthesis, and plant hormone signal transduction. These metabolic pathways are necessary for pearl millet to withstand and adapt to abiotic stresses caused by salt and heat. Moreover, the pearl millet shoot heat stress group showed specific transcriptomics related to KEEG metabolic pathways such as cytochrome P450, cutin, suberine, and wax biosynthesis, zeatin biosynthesis, crocin biosynthesis, ginsenoside biosynthesis, saponin biosynthesis, and biosynthesis of various plant secondary metabolites. In contrast, pearl millet shoots exposed to salinity stress exhibited transcriptomic changes associated with KEEG metabolic pathways related to carbon fixation in photosynthetic organisms, mismatch repair, and nitrogen metabolism. Our findings underscore the remarkable cross-tolerance of pearl millet to simultaneous salt and heat stress, elucidated through the activation of shared abiotic KEGG pathways. This study emphasizes the pivotal role of transcriptomics analysis in unraveling the molecular responses of pearl millet under abiotic stress conditions.
摘要：
珍珠小米（狼尾草（L.))是一种重要的谷类作物，以在充满挑战的环境条件下茁壮成长的能力而闻名；然而，控制其盐胁迫耐受性的分子机制仍然知之甚少。为了解决这个差距，进行了下一代RNA测序，以比较暴露于盐胁迫的珍珠小米幼苗与正常条件下生长的珍珠小米幼苗的基因表达模式。我们的RNA测序分析集中在经受盐度胁迫（150mmolNaCl3天）或热胁迫（50°C60s）的13天大的珍珠小米植物的芽上。在检查的36,041个基因中，成功鉴定了17,271个基因，其倍数变化范围为2.2至19.6。具体来说,2388个基因在响应热胁迫时差异上调，而4327个基因下调。在盐胁迫条件下，2013年基因上调，4221个基因下调。转录组学分析揭示了四种常见的非生物KEGG途径，这些途径在珍珠小米对盐和热胁迫的反应中起着至关重要的作用：苯丙烷生物合成，光合作用天线蛋白，光合作用,和植物激素信号转导。这些代谢途径是珍珠粟承受和适应由盐和热引起的非生物胁迫所必需的。此外，珍珠小米芽热胁迫组显示与KEEG代谢途径相关的特定转录组学，如细胞色素P450，角质，suberine,和蜡的生物合成，玉米素生物合成，藏红花素生物合成，人参皂苷生物合成，皂苷生物合成，和各种植物次生代谢产物的生物合成。相比之下,暴露于盐度胁迫的珍珠小米芽表现出与光合生物中与碳固定相关的KEEG代谢途径相关的转录组变化，失配修复,和氮代谢。我们的发现强调了珍珠小米对同时的盐和热胁迫的显着交叉耐受性，通过激活共享的非生物KEGG途径阐明。这项研究强调了转录组学分析在揭示珍珠小米在非生物胁迫条件下的分子响应中的关键作用。
