PDF(Pubmed)
Abstract：
The aim of this study was to molecularly investigate the presence of antimicrobial resistance genes (ARGs) in organ samples from 11 Apennine wolves (Canis lupus italicus) collected in Central Italy. Samples from lung, liver, spleen, kidney, tongue and intestine were investigated by PCRs targeting the following genes: tet(A), tet(B), tet(C), tet(D), tet(E), tet(G), tet(K), tet(L), tet(M), tet(O), tetA(P), tet(Q), tet(S), tet(X), sul1, sul2, sul3, blaCTX-M, blaSHV, blaTEM and mcr-1. A PCR positivity was highlighted for 13 out of the 21 tested genes; no positive results were obtained for tet(C), tet(D), tet(E), tet(G), sul3, blaCTX, blaSHV and mcr-1 genes. All 11 animals sampled showed positivity for one or more resistance genes. The results confirm the potential role of the wolf as an indicator and/or vector of antimicrobial-resistant bacteria or ARGs.
摘要：
这项研究的目的是通过分子调查从意大利中部收集的11只亚平宁狼（Canislupusitalicus）的器官样品中是否存在抗微生物抗性基因（ARG）。肺样本，肝脏,脾,脾肾,通过针对以下基因的PCR研究了舌头和肠道：tet(A)，tet(B),tet(C),tet(D),tet(E),tet(G),tet(K),tet(L),tet(M),tet(O),tetA(P),tet(Q),tet(S),tet(X),sul1,sul2,sul3,blaCTX-M,blaSHV,blaTEM和mcr-1。在21个测试基因中，有13个突出显示了PCR阳性；tet（C）未获得阳性结果，tet(D),tet(E),tet(G),sul3,blaCTX,blaSHV和mcr-1基因。取样的所有11只动物均显示一个或多个抗性基因的阳性。结果证实了狼作为抗微生物抗性细菌或ARGs的指示物和/或载体的潜在作用。
