Abstract：
Gold core mesoporous silica shell (AuMSS) nanorods are multifunctional nanomedicines that can act simultaneously as photothermal, drug delivery, and bioimaging agents. Nevertheless, it is reported that once administrated, nanoparticles can be coated with blood proteins, forming a protein corona, that directly impacts on nanomedicines\' circulation time, biodistribution, and therapeutic performance. Therefore, it become crucial to develop novel alternatives to improve nanoparticles\' half-life in the bloodstream. In this work, Polyethylenimine (PEI) and Red blood cells (RBC)-derived membranes were combined for the first time to functionalize AuMSS nanorods and simultaneously load acridine orange (AO). The obtained results revealed that the RBC-derived membranes promoted the neutralization of the AuMSS\' surface charge and consequently improved the colloidal stability and biocompatibility of the nanocarriers. Indeed, the in vitro data revealed that PEI/RBC-derived membranes\' functionalization also improved the nanoparticles\' cellular internalization and was capable of mitigating the hemolytic effects of AuMSS and AuMSS/PEI nanorods. In turn, the combinatorial chemo-photothermal therapy mediated by AuMSS/PEI/RBC_AO nanorods was able to completely eliminate HeLa cells, contrasting with the less efficient standalone therapies. Such data reinforce the potential of AuMSS nanomaterials to act simultaneously as photothermal and chemotherapeutic agents.
摘要：
金核介孔二氧化硅壳（AuMSS）纳米棒是多功能纳米药物，可以同时作为光热，药物输送,和生物成像剂。然而，据报道，一旦被管理，纳米粒子可以用血液蛋白包被，形成蛋白质日冕，直接影响纳米药物的循环时间，生物分布，和治疗性能。因此,开发新的替代品以改善纳米粒子在血液中的半衰期变得至关重要。在这项工作中,首次将聚乙烯亚胺（PEI）和红细胞（RBC）衍生的膜结合起来，以功能化AuMSS纳米棒并同时负载吖啶橙（AO）。获得的结果表明，RBC衍生的膜促进了AuMSS表面电荷的中和，从而改善了纳米载体的胶体稳定性和生物相容性。的确,体外数据显示，PEI/RBC衍生的膜功能化也改善了纳米颗粒的细胞内化，并能够减轻AuMSS和AuMSS/PEI纳米棒的溶血作用。反过来,AuMSS/PEI/RBC_AO纳米棒介导的联合化学-光热疗法能够完全消除HeLa细胞，与效率较低的独立疗法形成对比。这些数据增强了AuMSS纳米材料同时充当光热和化学治疗剂的潜力。
