Abstract:
The International Agency for Research on Cancer (IARC) classifies PFOA as a Class 1 carcinogen. Here, a new naked-eye PFOA immunochromographic strip was developed to recognize PFOA in domestic water and real human samples within 10 min based on a novel custom designed anti-PFOA monoclonal antibody (mAb) 2A3, which was firstly an immune rapid detection method for PFOA has been proposed. Using computer simulation techniques such as quantum computing to assist in designing the structural formula of PFOA semi antigen, which hapten was firstly proposed. The half maximal inhibitory concentration of PFOA monoclonal antibody (mAb) 2A3 was 2.4 μg/mL. Using mAb 2A3, we developed an immunochromatographic strip (ICS) for detecting PFOA in real samples. The developed method generated results in 10 min, with visual detection limits of 20, 20, and 200 μg/mL and limit of detection of 50, 200, and 500 μg/mL for water, blood and urine samples, respectively. The established ICS and indirect competitive enzyme-linked immunosorbent assay were used to analyze the actual samples, and the results were confirmed by LC-MS/MS. Our study findings showed that the ICS and ic-ELISA can quickly detect PFOA in actual samples.
摘要:
国际癌症研究机构(IARC)将PFOA归类为1类致癌物。这里,基于一种新颖的定制设计的抗PFOA单克隆抗体(mAb)2A3,开发了一种新的裸眼PFOA免疫染色试纸,可在10分钟内识别生活用水和真实人体样品中的PFOA,这首先是一种用于PFOA的免疫快速检测方法已被提出。利用量子计算等计算机模拟技术辅助设计PFOA半抗原的结构式,其中hapten是首次提出的。PFOA单克隆抗体(mAb)2A3的半数最大抑制浓度为2.4μg/mL。使用mAb2A3,我们开发了用于检测真实样品中的PFOA的免疫层析试纸条(ICS)。开发的方法在10分钟内产生结果,水的目测检出限为20、20和200μg/mL,检出限为50、200和500μg/mL,血液和尿液样本,分别。建立的ICS和间接竞争酶联免疫吸附试验用于分析实际样品,结果通过LC-MS/MS确认。我们的研究结果表明,ICS和ic-ELISA可以快速检测实际样品中的PFOA。