Abstract：
Previous vaccination trials have demonstrated that thiol proteins affinity purified from Ostertagia ostertagi excretory-secretory products (O. ostertagi ES-thiol) are protective against homologous challenge. Here we have shown that protection induced by this vaccine was consistent across four independent vaccine-challenge experiments. Protection is associated with reduced cumulative faecal egg counts across the duration of the trials, relative to control animals. To better understand the diversity of antigens in O. ostertagi ES-thiol we used high-resolution shotgun proteomics to identify 490 unique proteins in the vaccine preparation. The most numerous ES-thiol proteins, with 91 proteins identified, belong to the sperm-coating protein/Tpx/antigen 5/pathogenesis-related protein 1 (SCP/TAPS) family. This family includes previously identified O. ostertagi vaccine antigens O. ostertagi ASP-1 and ASP-2. The ES-thiol fraction also has numerous proteinases, representing three distinct classes, including: metallo-; aspartyl- and cysteine proteinases. In terms of number of family members, the M12 astacin-like metalloproteinases, with 33 proteins, are the most abundant proteinase family in O. ostertagi ES-thiol. The O. ostertagi ES-thiol proteome provides a comprehensive database of proteins present in this vaccine preparation and will guide future vaccine antigen discovery projects.
摘要：
先前的疫苗接种试验表明，从Ostertagiaostertagi排泄分泌产物（O.ostertagiES-硫醇）对同源攻击具有保护性。在这里，我们已经表明，这种疫苗诱导的保护在四个独立的疫苗攻击实验中是一致的。在整个试验过程中，保护与累积粪便卵数减少有关，相对于对照动物。为了更好地了解O.ostertagiES-硫醇中抗原的多样性，我们使用高分辨率shot弹枪蛋白质组学鉴定了疫苗制剂中的490种独特蛋白质。数量最多的ES-硫醇蛋白，鉴定出91种蛋白质，属于精子包被蛋白/Tpx/抗原5/发病相关蛋白1（SCP/TAPS）家族。该家族包括先前鉴定的O.ostertagi疫苗抗原O.ostertagiASP-1和ASP-2。ES-硫醇部分还具有许多蛋白酶，代表三个不同的类，包括:金属蛋白酶;天冬氨酰蛋白酶和半胱氨酸蛋白酶。从家庭成员的数量来看,M12胃酸样金属蛋白酶，有33种蛋白质，是O.ostertagiES-硫醇中最丰富的蛋白酶家族。O.ostertagiES-硫醇蛋白质组提供了该疫苗制剂中存在的蛋白质的全面数据库，并将指导未来的疫苗抗原发现项目。
