PDF(Pubmed)
Abstract：
BACKGROUND: Metazoan adenosine-to-inosine (A-to-I) RNA editing resembles A-to-G mutation and increases proteomic diversity in a temporal-spatial manner, allowing organisms adapting to changeable environment. The RNA editomes in many major animal clades remain unexplored, hampering the understanding on the evolution and adaptation of this essential post-transcriptional modification.
METHODS: We assembled the chromosome-level genome of Coridius chinensis belonging to Hemiptera, the fifth largest insect order where RNA editing has not been studied yet. We generated ten head RNA-Seq libraries with DNA-Seq from the matched individuals.
RESULTS: We identified thousands of high-confidence RNA editing sites in C. chinensis. Overrepresentation of nonsynonymous editing was observed, but conserved recoding across different orders was very rare. Under cold stress, the global editing efficiency was down-regulated and the general transcriptional processes were shut down. Nevertheless, we found an interesting site with \"conserved editing but non-conserved recoding\" in potassium channel Shab which was significantly up-regulated in cold, serving as a candidate functional site in response to temperature stress.
CONCLUSIONS: RNA editing in C. chinensis largely recodes the proteome. The first RNA editome in Hemiptera indicates independent origin of beneficial recoding during insect evolution, which advances our understanding on the evolution, conservation, and adaptation of RNA editing.
METHODS: We assembled the chromosome-level genome of Coridius chinensis belonging to Hemiptera, the fifth largest insect order where RNA editing has not been studied yet. We generated ten head RNA-Seq libraries with DNA-Seq from the matched individuals.
RESULTS: We identified thousands of high-confidence RNA editing sites in C. chinensis. Overrepresentation of nonsynonymous editing was observed, but conserved recoding across different orders was very rare. Under cold stress, the global editing efficiency was down-regulated and the general transcriptional processes were shut down. Nevertheless, we found an interesting site with \"conserved editing but non-conserved recoding\" in potassium channel Shab which was significantly up-regulated in cold, serving as a candidate functional site in response to temperature stress.
CONCLUSIONS: RNA editing in C. chinensis largely recodes the proteome. The first RNA editome in Hemiptera indicates independent origin of beneficial recoding during insect evolution, which advances our understanding on the evolution, conservation, and adaptation of RNA editing.
摘要：
背景：后生动物腺苷到肌苷（A到I）RNA编辑类似于A到G突变，并以时空方式增加蛋白质组多样性，让生物体适应多变的环境。许多主要动物进化枝的RNA编辑基因组仍未被探索，阻碍了对这种基本转录后修饰的进化和适应的理解。
方法：我们组装了属于半翅目的Coridiuschinensis的染色体水平基因组，还没有研究RNA编辑的第五大昆虫顺序。我们用来自匹配个体的DNA-Seq产生了十个头部RNA-Seq文库。
结果：我们在中国菜中发现了数千个高置信度的RNA编辑位点。观察到非同义词编辑的过度表达，但是在不同订单之间进行保守的重新编码非常罕见。在寒冷的压力下，全球编辑效率下调，一般转录过程被关闭。然而，我们在钾通道Shab中发现了一个有趣的“保守编辑但非保守重新编码”位点，该位点在寒冷时显著上调，作为响应温度胁迫的候选功能位点。
结论：在C.chinensis中的RNA编辑在很大程度上重新标记了蛋白质组。半翅目中的第一个RNAeditome表明了昆虫进化过程中有益重新编码的独立起源，这促进了我们对进化的理解，养护,和适应RNA编辑。
方法：我们组装了属于半翅目的Coridiuschinensis的染色体水平基因组，还没有研究RNA编辑的第五大昆虫顺序。我们用来自匹配个体的DNA-Seq产生了十个头部RNA-Seq文库。
结果：我们在中国菜中发现了数千个高置信度的RNA编辑位点。观察到非同义词编辑的过度表达，但是在不同订单之间进行保守的重新编码非常罕见。在寒冷的压力下，全球编辑效率下调，一般转录过程被关闭。然而，我们在钾通道Shab中发现了一个有趣的“保守编辑但非保守重新编码”位点，该位点在寒冷时显著上调，作为响应温度胁迫的候选功能位点。
结论：在C.chinensis中的RNA编辑在很大程度上重新标记了蛋白质组。半翅目中的第一个RNAeditome表明了昆虫进化过程中有益重新编码的独立起源，这促进了我们对进化的理解，养护,和适应RNA编辑。
