PDF(Pubmed)
Abstract：
Weight cycling is a major challenge in obesity management. Caloric restriction is known to promote this phenomenon, but the impact of macronutrient changes during dieting remains unclear. This study aimed to determine the role of macronutrient changes in weight maintenance without caloric restriction by alternating between two hypercaloric diets: a high-carbohydrate, high-fat Western diet (WD) and a low-carbohydrate, high-fat diet (LCHDF). Obesity was induced in 8-week-old C57BL/6 male mice by 10 weeks of WD feeding. Then, the mice were subjected to 12 weeks of LCHFD interspersed with WD (I-WD), 3 periods of 2-week LCHFD followed by 2 periods of 3-week WD, or 12 weeks of continuous WD (C-WD). C-WD and I-WD mice were compared to standard diet (SD) mice. In the I-WD group, each LCHFD period decreased weight gain, but mice regained weight after WD resumption. I-WD mice exhibited obesity, dyslipidemia, and glucose intolerance, similarly to the C-WD mice. I-WD mice also developed nonalcoholic steatohepatitis, associated with an increase in type-III collagen gene expression and a decrease in FGF21 protein levels, in comparison with SD. I-WD mice developed weight cycling despite maintaining a high caloric consumption, suggesting that changes in macronutrients during dieting are also a trigger of weight regain.
摘要：
体重循环是肥胖管理中的主要挑战。已知热量限制会促进这种现象，但节食过程中大量营养素变化的影响仍不清楚。这项研究旨在通过在两种高热量饮食之间交替进行，确定在没有热量限制的情况下维持体重的大量营养素变化的作用：高碳水化合物，高脂肪西方饮食（WD）和低碳水化合物，高脂肪饮食（LCHDF）。通过10周的WD喂养在8周龄C57BL/6雄性小鼠中诱导肥胖。然后，对小鼠进行12周的LCHFD穿插WD（I-WD），3个为期2周的LCHFD，然后是2个为期3周的WD，或连续12周的WD（C-WD）。将C-WD和I-WD小鼠与标准饮食(SD)小鼠进行比较。在I-WD组中,每个LCHFD周期都会减少体重增加，但WD恢复后小鼠体重恢复。I-WD小鼠表现出肥胖，血脂异常,和葡萄糖不耐受，与C-WD小鼠相似。I-WD小鼠也发展为非酒精性脂肪性肝炎，与III型胶原基因表达增加和FGF21蛋白水平降低相关，与SD相比。I-WD小鼠出现体重循环，尽管保持高热量消耗，这表明节食过程中大量营养素的变化也是体重恢复的触发因素。
