PDF(Pubmed)
Abstract：
Perirenal adipose tissue (PRAT) is a unique visceral depot that contains a mixture of brown and white adipocytes. The origin and plasticity of such cellular heterogeneity remains unknown. Here, we combine single-nucleus RNA sequencing with genetic lineage tracing to reveal the existence of a distinct subpopulation of Ucp1-&Cidea+ adipocytes that arises from brown-to-white conversion during postnatal life in the periureter region of mouse PRAT. Cold exposure restores Ucp1 expression and a thermogenic phenotype in this subpopulation. These cells have a transcriptome that is distinct from subcutaneous beige adipocytes and may represent a unique type of cold-recruitable adipocytes. These results pave the way for studies of PRAT physiology and mechanisms controlling the plasticity of brown/white adipocyte phenotypes.
摘要：
肾周脂肪组织（PRAT）是一种独特的内脏储库，含有棕色和白色脂肪细胞的混合物。这种细胞异质性的起源和可塑性仍然未知。这里,我们将单核RNA测序与遗传谱系追踪相结合，以揭示在小鼠PRAT的输尿管周围区域出生后生命期间由棕色至白色转化引起的Ucp1-和Cidea脂肪细胞的独特亚群的存在。冷暴露可恢复该亚群中的Ucp1表达和产热表型。这些细胞具有不同于皮下米色脂肪细胞的转录组,并且可能代表一种独特类型的冷可募集脂肪细胞。这些结果为研究PRAT生理学和控制棕色/白色脂肪细胞表型可塑性的机制铺平了道路。
