PDF(Pubmed)
Abstract：
Acute sleep deprivation upregulates hippocampal neurogenesis. Neurotrophic factors such as glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), and vascular endothelial growth factor (VEGF) are mediators of neuronal plasticity and neurogenesis. These neurotrophins are involved in sleep and sleep disorders and are associated with sleep deprivation. In this study, it is aimed to investigate the changes of neurotrophin levels with total sleep deprivation in healthy individuals. Seventeen healthy young adults with a mean age of 19.8 (SD = 1.0) years underwent an experimental protocol consisting of 36 h of total sleep deprivation. Venous blood samples were obtained on Day1 at 09.00, on Day2 at 09.00, and at 21.00. Serum levels of neurotrophins were detected using the ELISA method. The participants were asked to mark the scores corresponding to their subjective energy, happiness, depression, tension levels on the visual analog scale; and sleepiness level on the Epworth Sleepiness Scale; during the course of the study. As a result of 36 h of sleep deprivation, serum GDNF, BDNF, and VEGF levels showed a statistically significant increase compared to the baseline values in the participants included in the study (P < 0.0001). While this increase was evident in 24 h, it continued after 36 h. In parallel, sleepiness levels, subjective depression, and tension levels increased, on the other hand, subjective energy and happiness scores decreased at a statistically significant level at the end of the study compared to basal values (P < 0.0001). The results show that acute sleep deprivation significantly affects and increases serum levels of neurotrophic factors, and it seems that these effects are likely to occur as an immediate response to the stress and disruption caused by sleep deprivation.
摘要：
急性睡眠剥夺上调海马神经发生。神经营养因子，如胶质细胞源性神经营养因子（GDNF），脑源性神经营养因子(BDNF),血管内皮生长因子（VEGF）是神经元可塑性和神经发生的介质。这些神经营养蛋白与睡眠和睡眠障碍有关，并与睡眠剥夺有关。在这项研究中,目的探讨健康人总睡眠剥夺对神经营养因子水平的影响。17名平均年龄为19.8岁（SD=1.0）的健康年轻人接受了一项实验方案，其中包括36小时的总睡眠剥夺。在09.00的Day1、09.00的Day2和21.00获得静脉血样。使用ELISA方法检测血清神经营养因子水平。参与者被要求标记与他们的主观能量相对应的分数，幸福,抑郁症,视觉模拟量表上的紧张水平；和Epworth嗜睡量表上的嗜睡水平；在研究过程中。由于36小时的睡眠剥夺，血清GDNF,BDNF,与纳入研究的参与者的基线值相比，VEGF水平显示出统计学上的显着增加（P<0.0001）。虽然这种增加在24小时内很明显，它在36小时后继续。同时，困倦程度,主观抑郁,紧张程度增加，另一方面,与基础值相比,研究结束时的主观精力和幸福感得分在统计学上显著下降(P<0.0001).结果表明，急性睡眠剥夺显著影响和增加血清神经营养因子水平，似乎这些影响很可能是对睡眠不足造成的压力和干扰的即时反应。
