Abstract：
Ameloblastoma (AM) is characterised by local aggressiveness and bone resorption. To our knowledge, the proteomic profile of bone adjacent to AM has not previously been explored. We therefore looked at the differential proteins in cancellous bone (CB) adjacent to AM and normal CB from the mandible. CB proteins were extracted, purified, quantified, and analysed by liquid chromatography-mass spectrometry (LC-MS) using samples from five patients with AM. These proteins were further investigated using gene ontology for additional functional annotation and enrichment. Proteins that met the screening requirements of expression difference ploidy > 1.5-fold (upregulation and downregulation) and p < 0.05 were subsequently deemed differential proteins. Immunohistochemical staining was performed to confirm the above findings. Compared with normal mandibular CB, 151 differential proteins were identified in CB adjacent to the mandibular AM. These were mainly linked to cellular catabolic processes, lipid metabolism, and fatty acids (FA) metabolism. LC-MS and immunohistochemistry showed that CD36 was one of the notably decreased proteins in CB bordering the AM compared with normal mandibular CB (p = 0.0066 and p = 0.0095, respectively). CD36 expression in CB correlates with bone remodelling in AM, making CD36 a viable target for therapeutic approaches.
摘要：
成釉细胞瘤(AM)的特征在于局部侵袭性和骨吸收。据我们所知,先前尚未探索与AM相邻的骨的蛋白质组学特征。因此，我们从下颌骨观察了与AM和正常CB相邻的松质骨（CB）中的差异蛋白。提取CB蛋白,纯化,量化,并使用来自五名AM患者的样品通过液相色谱-质谱（LC-MS）进行分析。使用基因本体论进一步研究这些蛋白质以进行额外的功能注释和富集。满足表达差异倍性>1.5倍(上调和下调)和p<0.05的筛选要求的蛋白质随后被认为是差异蛋白质。进行免疫组织化学染色以证实上述发现。与正常下颌CB相比，在下颌AM附近的CB中鉴定出151种差异蛋白。这些主要与细胞分解代谢过程有关，脂质代谢，和脂肪酸（FA）代谢。LC-MS和免疫组织化学显示，与正常下颌CB相比，CD36是与AM接壤的CB中明显减少的蛋白质之一（分别为p=0.0066和p=0.0095）。CB中CD36的表达与AM中的骨重塑相关，使CD36成为治疗方法的可行靶标。
