Abstract：
Cryptosporidium spp., Enterocytozoon bieneusi and Encephalitozoon spp. are the most common protistan parasites of vertebrates. The results show that pigeon populations in Central Europe are parasitised by different species of Cryptosporidium and genotypes of microsporidia of the genera Enterocytozoon and Encephalitozoon. A total of 634 and 306 faecal samples of captive and feral pigeons (Columba livia f. domestica) from 44 locations in the Czech Republic, Slovakia and Poland were analysed for the presence of parasites by microscopy and PCR/sequence analysis of small subunit ribosomal RNA (18S rDNA), 60 kDa glycoprotein (gp60) and internal transcribed spacer (ITS) of SSU rDNA. Phylogenetic analyses revealed the presence of C. meleagridis, C. baileyi, C. parvum, C. andersoni, C. muris, C. galli and C. ornithophilus, E. hellem genotype 1A and 2B, E. cuniculi genotype I and II and E. bieneusi genotype Peru 6, CHN-F1, D, Peru 8, Type IV, ZY37, E, CHN4, SCF2 and WR4. Captive pigeons were significantly more frequently parasitised with screened parasite than feral pigeons. Cryptosporidium meleagridis IIIa and a new subtype IIIl have been described, the oocysts of which are not infectious to immunodeficient mice, whereas chickens are susceptible. This investigation demonstrates that pigeons can be hosts to numerous species, genotypes and subtypes of the studied parasites. Consequently, they represent a potential source of infection for both livestock and humans.
摘要：
隐孢子虫。,肠孢子虫和头孢菌素。是脊椎动物中最常见的protistan寄生虫。结果表明，中欧的鸽子种群被不同种类的隐孢子虫和肠孢子虫属和头孢菌素属的微孢子虫的基因型所寄生。来自捷克共和国44个地点的圈养鸽子和野鸽（Columbaliviaf.domestica）的粪便样本共634和306个，通过显微镜和小亚基核糖体RNA（18SrDNA）的PCR/序列分析，分析了斯洛伐克和波兰是否存在寄生虫，SSUrDNA的60kDa糖蛋白(gp60)和内部转录间隔区(ITS)。系统发育分析显示存在meleagridis，C.Baileyi,C.parvum,C.Andersoni,C.Muris,C.Galli和C.E.hellem基因型1A和2B，E.cuniculi基因型I和II和E.bieneusi基因型秘鲁6，CHN-F1，D，秘鲁8,IV型,ZY37,E,CHN4、SCF2和WR4。圈养的鸽子被筛选的寄生虫寄生的频率明显高于野鸽。已经描述了隐孢子虫IIIa和新的亚型IIIl，其中的卵囊对免疫缺陷小鼠没有传染性，而鸡是易感的。这项调查表明，鸽子可以成为许多物种的宿主，所研究寄生虫的基因型和亚型。因此,它们代表了牲畜和人类的潜在感染源。
