国内育中鸽胸肌发育中基因调控的机制仍然难以捉摸。这里,我们使用来自国内榆中鸽子和欧洲肉鸽的Iso-seq和RNA-seq数据进行了比较分析,以揭示与胸肌发育有关的信号通路和基因。来自国内榆中鸽子的Iso-seq数据产生了131,377,075个亚读,导致16,587个非冗余高质量全长转录本校正后。此外,利用pfam,CPC,PLEK,和CPAT,我们预测了5575、4973、2333和4336个lncRNAs,分别。值得注意的是,鉴定了几种可能与胸肌发育有关的基因,包括原肌球蛋白β链,肌球蛋白调节轻链2和肌球蛋白结合蛋白C。KEGG富集分析揭示了乳肌发育中的关键信号通路,跨越碳代谢,氨基酸的生物合成,糖酵解/糖异生,雌激素信号,PI3K-AKT信号,内质网中的蛋白质加工,氧化磷酸化,戊糖磷酸途径,果糖和甘露糖代谢,和紧密连接。这些发现提供了对驱动国内榆中鸽子乳房肌肉发育的生物过程的见解,有助于我们理解这个复杂的现象。
The mechanisms governing gene regulation in domestic Yuzhong pigeon breast muscle development remain largely elusive. Here, we conducted a comparative analysis using Iso-seq and RNA-seq data from domestic Yuzhong pigeons and European meat pigeons to uncover signaling pathways and genes involved in breast muscle development. The Iso-seq data from domestic Yuzhong pigeons yielded 131,377,075 subreads, resulting in 16,587 non-redundant high-quality full-length transcripts post-correction. Furthermore, utilizing pfam, CPC, PLEK, and CPAT, we predicted 5575, 4973, 2333, and 4336 lncRNAs, respectively. Notably, several genes potentially implicated in breast muscle development were identified, including tropomyosin beta chain, myosin regulatory light chain 2, and myosin binding protein C. KEGG enrichment analysis revealed critical signaling pathways in breast muscle development, spanning carbon metabolism, biosynthesis of amino acids, glycolysis/gluconeogenesis, estrogen signaling, PI3K-AKT signaling, protein processing in the endoplasmic reticulum, oxidative phosphorylation, pentose phosphate pathway, fructose and mannose metabolism, and tight junctions. These findings offer insights into the biological processes driving breast muscle development in domestic Yuzhong pigeon, contributing to our understanding of this complex phenomenon.