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Abstract：
BACKGROUND: In this study, the protective immunity and immunogenicity of the monovalent and bivalent Streptococcus iniae and Vibrio harveyi vaccine were evaluated in Asian seabass. To analyze immune responses, 1200 Asian seabass with an average weight of 132.6 ± 25.4 g were divided into eight treatments in triplicates (50 fish per tank) as follows: S. iniae immunized by injection (SI), V. harveyi immunized by injection (VI), bivalent S. iniae and V. harveyi (SVI) immunized by injection, S. iniae immunized by immersion (SIM), V. harveyi (VIM) immunized by immersion, bivalent S. iniae and V. harvei (SVIM) immunized by immersion, phosphate-buffered saline (PBS) by injection (PBSI) and control group without vaccine administration (CTRL). Blood and serum samples were taken at the end of the 30th and 60th days. Then the vaccinated groups were challenged with two bacteria (S. iniae) and (V. harveyi) separately and mortality was recorded for 14 days.
RESULTS: This study reveals that there is no significant difference in the hematological parameters on the 30th and 60th days of the experiment in the vaccine-immunized groups compared to the CTRL group (P > 0.05). Meanwhile, there was no significant difference in the amount of serum albumin level, respiratory burst activity, and serum bactericidal activity in the vaccine-immunized groups compared to the CTRL group on the 30th and 60th days of the experiment (P > 0.05). Total protein on the 60th day (in the VI and SVI groups), globulin on the 30th day (in the VI and SVI groups) and the 60th day (in the VI group) compared to the CTRL and PBSI groups had a significant increase (P < 0.05). Complement activity (in the VI and SVI groups) and lysozyme (in the SI and SVI groups) increased significantly compared to the control group (P < 0.05). Serum antibody titer against S. iniae had a significant increase in the SI, VI, SVI and SVIM groups compared to the CTRL and PBSI groups (P < 0.05). Serum antibody titer against V. harveyi had a significant increase in the groups immunized with the vaccine compared to the CTRL and PBSI groups (P < 0.05). A significant increase in the relative percentage survival (RPS) following challenge with S. iniae in the SVI (86.6%), SI (83.3%,) and VI (73.3%) groups were observed compared to the CTRL (43.3%) and PBSI (40%) groups (P < 0.05). Also, a significant increase in the RPS after challenge with V. harveyi in the SVI group, VI 86.6%, SVI 83.3%, VIM 80% and SVIM 76.6% were observed compared to the CTRL (46.6%) and PBSI (50%) groups (P < 0.05).
CONCLUSIONS: Overall, the results demonstrated that the bivalent vaccine of S. iniae and V. harveywas able to produce significant immunogenicity and RPS in Asian seabass.
RESULTS: This study reveals that there is no significant difference in the hematological parameters on the 30th and 60th days of the experiment in the vaccine-immunized groups compared to the CTRL group (P > 0.05). Meanwhile, there was no significant difference in the amount of serum albumin level, respiratory burst activity, and serum bactericidal activity in the vaccine-immunized groups compared to the CTRL group on the 30th and 60th days of the experiment (P > 0.05). Total protein on the 60th day (in the VI and SVI groups), globulin on the 30th day (in the VI and SVI groups) and the 60th day (in the VI group) compared to the CTRL and PBSI groups had a significant increase (P < 0.05). Complement activity (in the VI and SVI groups) and lysozyme (in the SI and SVI groups) increased significantly compared to the control group (P < 0.05). Serum antibody titer against S. iniae had a significant increase in the SI, VI, SVI and SVIM groups compared to the CTRL and PBSI groups (P < 0.05). Serum antibody titer against V. harveyi had a significant increase in the groups immunized with the vaccine compared to the CTRL and PBSI groups (P < 0.05). A significant increase in the relative percentage survival (RPS) following challenge with S. iniae in the SVI (86.6%), SI (83.3%,) and VI (73.3%) groups were observed compared to the CTRL (43.3%) and PBSI (40%) groups (P < 0.05). Also, a significant increase in the RPS after challenge with V. harveyi in the SVI group, VI 86.6%, SVI 83.3%, VIM 80% and SVIM 76.6% were observed compared to the CTRL (46.6%) and PBSI (50%) groups (P < 0.05).
CONCLUSIONS: Overall, the results demonstrated that the bivalent vaccine of S. iniae and V. harveywas able to produce significant immunogenicity and RPS in Asian seabass.
摘要：
背景：在这项研究中，在亚洲鲈鱼中评估了单价和二价链球菌和哈维氏弧菌疫苗的保护性免疫和免疫原性。为了分析免疫反应，平均体重为132.6±25.4g的1200亚洲鲈鱼被分为八种处理，一式三份（每缸50条鱼）如下：通过注射（SI）免疫的S.iniae，V.注射免疫harveyi（VI），通过注射免疫的二价S.iniae和V.harveyi（SVI），通过浸没(SIM)免疫的美国免疫组织，V.harveyi（VIM）通过浸没免疫，通过浸没免疫的二价S.iniae和V.harvei（SVIM），磷酸盐缓冲盐水(PBS)通过注射(PBSI)和对照组不施用疫苗(CTRL)。在第30天和第60天结束时采集血液和血清样品。然后用两种细菌(S.iniae)和(V.harveyi）分别记录死亡率14天。
结果：本研究显示，与CTRL组相比，疫苗免疫组实验第30天和第60天的血液学参数无显著差异（P>0.05）。同时,血清白蛋白水平没有显着差异，呼吸爆发活动，实验第30天和第60天疫苗免疫组血清杀菌活性与CTRL组比较(P>0.05)。第60天的总蛋白（VI和SVI组），球蛋白在第30天(在VI和SVI组)和第60天(在VI组)较CTRL和PBSI组有显著的增加(P<0.05)。补体活性(在VI和SVI组)和溶菌酶(在SI和SVI组)与对照组相比显著增加(P<0.05)。抗S.iniae的血清抗体滴度在SI中有显着增加，VI,SVI和SVIM组与CTRL和PBSI组比拟(P<0.05)。与CTRL和PBSI组相比，用疫苗免疫的组针对哈氏弧菌的血清抗体滴度显着增加（P<0.05）。在SVI中使用S.iniae攻击后，相对存活率（RPS）显着增加（86.6％），SI（83.3%，）和VI（73.3％）组与CTRL（43.3％）和PBSI（40％）组相比（P<0.05）。此外，在SVI组中与V.harveyi挑战后RPS显着增加，VI86.6%，SVI83.3%，与CTRL（46.6％）和PBSI（50％）组相比，观察到VIM80％和SVIM76.6％（P<0.05）。
结论：总体而言，结果表明，S.iniae和V.harve的二价疫苗能够在亚洲鲈鱼中产生显着的免疫原性和RPS。
结果：本研究显示，与CTRL组相比，疫苗免疫组实验第30天和第60天的血液学参数无显著差异（P>0.05）。同时,血清白蛋白水平没有显着差异，呼吸爆发活动，实验第30天和第60天疫苗免疫组血清杀菌活性与CTRL组比较(P>0.05)。第60天的总蛋白（VI和SVI组），球蛋白在第30天(在VI和SVI组)和第60天(在VI组)较CTRL和PBSI组有显著的增加(P<0.05)。补体活性(在VI和SVI组)和溶菌酶(在SI和SVI组)与对照组相比显著增加(P<0.05)。抗S.iniae的血清抗体滴度在SI中有显着增加，VI,SVI和SVIM组与CTRL和PBSI组比拟(P<0.05)。与CTRL和PBSI组相比，用疫苗免疫的组针对哈氏弧菌的血清抗体滴度显着增加（P<0.05）。在SVI中使用S.iniae攻击后，相对存活率（RPS）显着增加（86.6％），SI（83.3%，）和VI（73.3％）组与CTRL（43.3％）和PBSI（40％）组相比（P<0.05）。此外，在SVI组中与V.harveyi挑战后RPS显着增加，VI86.6%，SVI83.3%，与CTRL（46.6％）和PBSI（50％）组相比，观察到VIM80％和SVIM76.6％（P<0.05）。
结论：总体而言，结果表明，S.iniae和V.harve的二价疫苗能够在亚洲鲈鱼中产生显着的免疫原性和RPS。
