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Abstract：
Aureobasidium pullulans is a ubiquitous fungus with a wide variety of morphologies and growth modes including \"typical\" single-budding yeast, and interestingly, larger multinucleate yeast than can make multiple buds in a single cell cycle. The study of A. pullulans promises to uncover novel cell biology, but currently tools are lacking to achieve this goal. Here, we describe initial components of a cell biology toolkit for A. pullulans, which is used to express and image fluorescent probes for nuclei as well as components of the cytoskeleton. These tools allowed live-cell imaging of the multinucleate and multibudding cycles, revealing highly synchronous mitoses in multinucleate yeast that occur in a semiopen manner with an intact but permeable nuclear envelope. These findings open the door to using this ubiquitous polyextremotolerant fungus as a model for evolutionary cell biology.
摘要：
出芽梭菌是一种无处不在的真菌，具有多种形态和生长模式，包括“典型的”单芽酵母，有趣的是，更大的多核酵母比可以在一个细胞周期中产生多个芽。普鲁兰的研究有望发现新的细胞生物学，但是目前缺乏实现这一目标的工具。这里,我们描述了普鲁兰杆菌的细胞生物学工具包的初始组件，用于表达和成像细胞核以及细胞骨架成分的荧光探针。这些工具允许多核和多芽周期的活细胞成像，揭示多核酵母中高度同步的有丝分裂，以半开放的方式发生，具有完整但可渗透的核包膜。这些发现为使用这种普遍存在的多极耐受真菌作为进化细胞生物学模型打开了大门。[媒体:见文本][媒体:见文本][媒体:见文本][媒体:见文本]。
