{Reference Type}: Journal Article
{Title}: Tools for live-cell imaging of cytoskeletal and nuclear behavior in the unconventional yeast, Aureobasidium pullulans.
{Author}: Petrucco CA;Crocker AW;D'Alessandro A;Medina EM;Gorman O;McNeill J;Gladfelter AS;Lew DJ;
{Journal}: Mol Biol Cell
{Volume}: 35
{Issue}: 4
{Year}: 2024 Apr 1
{Factor}: 3.612
{DOI}: 10.1091/mbc.E23-10-0388
{Abstract}: Aureobasidium pullulans is a ubiquitous fungus with a wide variety of morphologies and growth modes including "typical" single-budding yeast, and interestingly, larger multinucleate yeast than can make multiple buds in a single cell cycle. The study of A. pullulans promises to uncover novel cell biology, but currently tools are lacking to achieve this goal. Here, we describe initial components of a cell biology toolkit for A. pullulans, which is used to express and image fluorescent probes for nuclei as well as components of the cytoskeleton. These tools allowed live-cell imaging of the multinucleate and multibudding cycles, revealing highly synchronous mitoses in multinucleate yeast that occur in a semiopen manner with an intact but permeable nuclear envelope. These findings open the door to using this ubiquitous polyextremotolerant fungus as a model for evolutionary cell biology.