PDF(Pubmed)
Abstract：
Haberlea rhodopensis, a resurrection species, is the only plant known to be able to survive multiple extreme environments, including desiccation, freezing temperatures, and long-term darkness. However, the molecular mechanisms underlying tolerance to these stresses are poorly studied. Here, we present a high-quality genome of Haberlea and found that ~ 23.55% of the 44,306 genes are orphan. Comparative genomics analysis identified 89 significantly expanded gene families, of which 25 were specific to Haberlea. Moreover, we demonstrated that Haberlea preserves its resurrection potential even in prolonged complete darkness. Transcriptome profiling of plants subjected to desiccation, darkness, and low temperatures revealed both common and specific footprints of these stresses, and their combinations. For example, PROTEIN PHOSPHATASE 2C (PP2C) genes were substantially induced in all stress combinations, while PHYTOCHROME INTERACTING FACTOR 1 (PIF1) and GROWTH RESPONSE FACTOR 4 (GRF4) were induced only in darkness. Additionally, 733 genes with unknown functions and three genes encoding transcription factors specific to Haberlea were specifically induced/repressed upon combination of stresses, rendering them attractive targets for future functional studies. The study provides a comprehensive understanding of the genomic architecture and reports details of the mechanisms of multi-stress tolerance of this resurrection species that will aid in developing strategies that allow crops to survive extreme and multiple abiotic stresses.
摘要：
Haberlearhodopensis,一个复活的物种，是已知唯一能够在多种极端环境中生存的植物，包括干燥，冻结温度,和长期的黑暗。然而,对这些应力耐受性的分子机制研究甚少。这里,我们提出了一个高质量的Haberlea基因组，发现44,306个基因中的〜23.55％是孤儿。比较基因组学分析确定了89个显著扩展的基因家族，其中25个是Haberlea特有的。此外，我们证明，即使在长时间的完全黑暗中，哈伯利亚也能保留其复活潜力。经历干燥的植物的转录组分析，黑暗,低温揭示了这些应力的共同和特定足迹，和他们的组合。例如,蛋白质磷酸酶2C（PP2C）基因在所有胁迫组合中基本上被诱导，而植物铬相互作用因子1（PIF1）和生长反应因子4（GRF4）仅在黑暗中诱导。此外,733个功能未知的基因和三个编码Haberlea特异性转录因子的基因在压力组合时被特异性诱导/抑制，使它们成为未来功能研究的有吸引力的目标。该研究提供了对基因组结构的全面了解，并报道了这种复活物种的多胁迫耐受性机制的详细信息，这将有助于制定使作物能够在极端和多种非生物胁迫下生存的策略。
